Abstract:

objective: To observe the effect of rescued mutant CIAV on the host cell cycle and apoptosis of MDCC-MSB1 through flow cytometry. Methods: MDCC-MSB1 was inoculated with rescued mutant CIAV M strain after 24 hours or 48 hours, while the negative control and the positive control were established. Cell cycle phase and apoptosis of MSB1 was detected by flow cytometry(FCM) with PI staining and FITC Annexin V staining. The acquirement was analysis by SPSS 13.0. Results: The percentage of G0/G1 phase of MDCC-MSB1 cultured with rescued mutant CIAV M strain was 59.33%±4.04%，the percentage of S phase was 32.92%±3.65% and that of G2/M phase was 7.75%±3.11%. The percentage of G0/G1 phase of the test group was larger than that of the negative control, in contrast to the positive control, however the differences were not markable(P>0.05). The apoptosis rate of MDCC-MSB1 cultured with rescued mutant CIAV M strain was 37.00% ± 4.65%, higher than that of negative control, while lower than the positive group, and the differences were significant(P <0.05). Conclusion: The results showed that the inhibition of the host cell cycle by rescued mutant CIAV M strain was not noticeable. The apoptosis of the host cell induced by vp3 gene mutation of CIAV was obvious. The consequence intimated that the vp3 gene mutation of CIAV reserved some characteristics of the virus pathogenicity, which can cause the lower apoptosis rate of cells compared to that of CUX-1, and the fundament theory was established through this study.