Hybrid Purity Identification of‘Xinong No.18’ by SSR Markers

doi:10.11924/j.issn.1000-6850.2012-1613

Abstract

Abstract: In order to establish a rapid and reliable SSR method to identify the purity of hybrids‘Xinong No. 18’, and to provide auxiliary tools to improve the field purity of‘Xinong No.18’. In this paper, the Brassica napus. hybrid varieties‘Xinong 18’and its parents are the materials. With SSR molecular marker was used to analysis the polymorphism of these materials, in order to screen the significantly Different primer between‘XinongNo.18’and its parents and identify the purity of‘Xinong No.18’with it. The results showed that, one pair of primer BRAS023 in 200 SSR primers was found had significant and legible polymorphism between hybrid seeds and parental inbred lines, and the polymorphism presented in the form that all the bands amplified from the hybrid seeds were complementary to those from their parents. 150 individual seeds were tested with the primer, and the seed purity was 86%, which was closed to the result identified in the field 89%. The results indicated SSR primer BRAS023 could be used to detect the purity of‘Xinong No.18’.

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