Abstract: The content of organic matter, available nitrogen, available phosphorous, available potassium of ancient tea garden soil nutrient were detected with oil bath heating potassium dichromate oxidation-dissolved method, alkali solution diffusion method and etc. in Jingmaishan and Liudachashan in order to make clear soil nutrient condition. Then, it provided the scientific basis for better protection and utilization of ancient tea garden. The results showed that the average content of the organic matter and available nitrogen of ancient tea gardens soil were higher in Jingmaishan than Liudachashan’s, but the average content of the available phosphorus and available potassium were lower in Jingmaishan than Liudachashan’s. The soil reach tea plantation soil standard ofⅠgrade in Jingmaishan ancient tea garden and Ⅱgrade in Liudachashan ancient tea garden. The coefficient of variation of the ancient tea garden soil nutrient content were available phosphorous, available nitrogen, available potassium and organic matter from large to small in Jingmaishan. The coefficient of variation of the ancient tea garden soil nutrient content were available phosphorous, available potassium, available nitrogen and organic matter from large to small in Liudachashan. The organic matter and available nitrogen were larger in jingmaishan than Liudachashan’s among coefficient of variation of soil nutrient, but available phosphorous and available potassium were smaller in Jingmaishan than Liudachashan’s. The LSD multiple comparison results showed that the average contents were different significant with organic matter, available nitrogen, available phosphorous, available potassium of sixteen locations ancient tea gardens in Jingmaishan and Liudachashan.