Abstract: The study was conducted to reveal the GRα mRNA expression in different tissues of gilts during late gestation in high ambient temperature. The object of this study was to investigate the relationship between the GRα mRNA expression and physiological development of gilts during late gestation, to provide the basic reference data for the study of molecular mechanisms of heat stress. Twelve gilts (Landrace × Large White) during late gestation were assigned into two treatments (6 gilts/ treatment) according to the gestational day (90d and 110d). The gilts were slaughtered and tissues including brain, ovary, heart, liver, spleen, lung and kidney were collected aseptically over liquid nitrogen and frozen at -80°C. Then total RNA isolation and reverse transcription, quantitative real-time PCR(qRT-PCR) was performed to determine the mRNA expression of GRα in different tissues of gilts during late gestation. The results showed that the mRNA expression level of GRα in different tissues of gilts at d 90 of gestation was as follows: lung > spleen > liver > kidney > brain > ovary > heart; d 110: spleen > lung > liver > kidney > ovary > heart > brain. Compared d 110 with d 90 of gestation, except that the expression of GRα in lung and brain was significantly reduced (P<0.01), and was reduced (P<0.05) in liver, there were no gestation stage dependent effects on GRα mRNA expression in all the other tissues (P>0.05). Our results indicated that the mRNA expression of GRα was higher in lung and spleen of gilts during late gestation, to a lesser degree in liver and kidney, lower expression levels of GRα were observed in brain, ovary and heart. Compared d 110 with d 90 of gestation, the expression of GRα mRNA were reduced in lung, brain and liver at different degrees. this changes with prolonged pregnancy is more obvious, but at the same time showed a distinct tissue specificity.