Abstract: Based on the measured results of the known area graphics and the leaves of Spiraea vanhouttei (Briot) Zabel., Primula ranunculoides Chen and Cayratia japonica (Thunb.) Gagn., the accuracy and repeatability of the two commonly used leaf area measurement methods (the paper-cut weighing method and the image pixels method) were tested. The analysis of two methods measured results of known area graphics indicated that the accuracy and repeatability of the two methods were good, and the relative error of both methods was less than 3%. But the accuracy and repeatability of the image pixels method were comparatively better than the paper-cut weighing method. Comparison two methods measured results of S. vanhouttei, P. ranunculoides and C. japonica revealed that the variation range of results measured by image pixels method were significantly less than that of paper-cut weighing method, and the comparability of the two methods measured results was decreased in smaller area leaves. Therefore, when in the measurement of leaves with small area, the image pixels method was recommended to use and the measured results will be more reliable.