Abstract: The potassium nutritional characteristics of five sugarcane genotypes were studied using tissue culture technology. The results showed that five genotypes had different potassium nutritional characteristics. With the concentration of potassium in culture medium in ranged of 0 ~ 30 mmol/L, the net fresh weight increase (NFWI) of YT60 and YT55 were highest at 15 mmol/L, while ROC22, YT236 and YY618 reached the maximum at 20 mmol/L. However, the net dry weight increase (NDWI) of five genotypes all reached the maximum at 20 mmol/L. Under low-K level (with the potassium concentration in range of 0~0.2 mmol/L), the NFWI and NDWI of YT60 and YT55 were significantly higher than YT236 and YY618. In addition, the potassium content and potassium accumulation in plantlets of each genotype increased with the increase of potassium concentration in the medium.