Abstract: The total RNA from petals of Camellia sasanqua was extracted by the methods of CTAB-many-level-precipitation method, improved isothiocyanate method, improved hot-borate method, Trizol method and EASY spin plant RNA extraction Kit method, respectively, and the five methods were contrasted and analyzed. The results showed that the method of EASY spin plant RNA extraction Kit was the simplest, the quickest and high efficient, and CTAB-many-level-precipitation method took the second place. The study provided convenient and effective technical supports for the research on molecular biology of Camellia sasanqua, and also provided necessary reference for the RNA extraction of other flowers.