Abstract: Analysis on polymorphism of CAST gene in Gansu Local Cattle and its relationship with meat quality trait. Polymorphisms in CAST gene were identified with PCR-RFLP analysis in Gansu local cattle. The association between genotype markers and meat traits were estimated by least squares using a linear model that included effects of locus and genotype appropriate interactions. The results showed that Polymorphisms of the CAST gene were evidenced three SNP locus T750C, A557G ,G556T, and 351 InDelGTT T/C mutation. Analysis of CAST gene in the Hga I locus there was little evidence of genotypic differences in meat quality traits. Analysis of CAST gene in the Hpy188 III locus these cattles with the AT genotype had a greater shear force than that of AA. Analysis of CAST gene in the Acl I locus these cattles with the Hh genotype had a greater shear force than that of HH. Cattle with the HH genotype had a greater pressing loss percentage than that of hh. There was little evidence of genotypic differences in meat quality traits. CAST gene were established the theory of a candidate molecular marker for meat quality trait.