Abstract: Youzhou Black Goat, confirmed to be a national genetic resource by the ovine committee in National Commission for Livestock and Poultry Genetic Resources in 2009, was characterized by black skin and flesh and thus has been highly regarded. Here, we examined the 12 blood biochemical indicators of this special goat to evaluate its germplasm characteristics. We demonstrated that the enzymatic activities of serum TP and GLOB in 3-4 years old goats were significantly higher than those in younger animals (P<0.05), and no differences were observed between 0-1, 1-2, and 2-3 years old team (P>0.05). There were no significant differences in each biochemical indicator between male and female (P>0.05). Correlation analysis of biochemical indicators suggests that there was markedly positive correlation between some indicators (P<0.01 or P<0.05 ), such as ALT/AST, ALT/ALB, ALT/TC, ALT/HDL-C, ALT/CK, and ALT/LDH, et al. Whereas, there was significant negative correlation between other indicators(P<0.01 or P<0.05), such as TP/A or G, GLOB/ A or G, and GLOB/LDL-C. As for relationship between biochemical indicators and phenotypes, the results indicated that ALT, TG, and LDL-C seem to be negative indicators fot body length, while LDL-C and ALT appear to be also negative indicators for chest circumference and femal body weight, respectively. The blood biochemical indicators could be used for evaluation of health as well as reference for animal breeding in future.