Abstract: A full-length cDNA of β-tubulin was obtained from plum (Prunus salicina Lindl var. cordata JY Zhang et al cv. Younai) fruit by means of RT-PCR and RACE. The complete cDNA was 1606bp in length, contained an opening reading frame of 1341bp encoding a peptide of 446 amino acid residues. The deduced amino acid sequence has high identity to those of many other plants. Specific primers were designed, semi-quantitative RT-PCR was performed, and the results showed that the expression of this gene was constant at 11 of different stages of fruit development, suggesting its availability for reference gene in the subsequent investigation on the expression of development-related genes in plum fruit using real-time quantitative PCR.