Molecular Cloning and Bioinformatics Analysis of SFB Genes Controlling Self-incompatibility in Xinjiang Wild Almond (Prunus tenella Batsch.)

doi:10.11924/j.issn.1000-6850.casb16100096

Abstract

Abstract: The study aims to clone full-length sequence of the new pollen SFB gene of self-incompatibility in Prunus tenella. Xinjiang wild almond was used as the material, and molecular homologous cloning and RT-PCR were conducted in this study. The online software was used to analyze the protein physicochemical properties of SFB. The full-length gene sequences of PtSFB5 and PtSFB6 were successfully cloned. The full-length sequence of the two SFB genes was 1124 bp and 1072 bp, respectively, and encoded 374 aa and 348 aa, respectively. Both of the two genes had F- Box structure, belonged to the F- Box gene family. The predicted relative molecular mass was 30787.26 and 44037.74, and the isoelectric point was 5.93 and 5.43, respectively. PtSFB5 and PtSFB6 protein belonged to the unstable water-soluble protein.

CLC Number:

S662.9

References

[1]李晓,张绍铃,吴俊,等. 樱桃品种S基因型及自交不亲和性分子机制研究进展[J]. 生物技术通报.2006(6):28-33.
[2]张剑锋. 甘蓝型油菜自交不亲和保持机理及AtPUB2在自交不亲和反应中功能研究[D]. 武汉:华中农业大学,2014:01.
[3]阳晶. 沙田柚花柱自交不亲和相关基因的克隆与鉴定[D]. 桂林:广西师范大学,2008:04.
[4]杨谷良,谭晓风,乌云塔娜. 配子体自交不亲和植物花粉S基因研究进展[J]. 中国生物工程杂志,2005,10:68-71 77.
[5]高启明. 新疆栽培扁桃自交不亲和性的研究[D].乌鲁木齐:新疆农业大学,2011:02.
[6]安雯,曹阳,李俞涛,等. 植物自交不亲和性的研究进展与展望[J]. 信阳师范学院学报:自然科学版,2009,22(04):630-634.
[7]孟冬. 苹果MdABCF转运S-RNase至花粉管影响自交不亲和反应[D]. 北京:中国农业大学,2014:01.
[8]徐义流,张绍铃. 梨配子体型自交不亲和性及其分子机理[J]. 果树学报,2003,20(01):59-63.
[9]王爱华,戴洪义. 果树自交不亲和性的研究进展[J]. 莱阳农学院学报,2002,19(03):206-209,220.
[10]曾斌,罗淑萍,李疆,等. 新疆野扁桃天然居群形态变异的研究[J]. 生物多样性,2008,16(05):484-491.
[11]吕志江,李疆,吾买尔夏提.塔汉,等. 新疆野扁桃种质资源遗传多样性的ISSR分析[J]. 果树学报,2010,06:918-923.
[12]宋宏伟；新疆野扁桃坚果品质鉴定和抗旱性研究[D].乌鲁木齐,新疆农业大学,2013.7
[13]郭仲军,李行斌,汪志军,等. 新疆野巴旦杏[J]. 中国野生植物资, 2002,21(01):21-61.
[14]李宁. 新疆野扁桃AlsCBF基因的克隆及功能验证[D]. 乌鲁木齐: 新疆农业大学,2012:01.
[15]曾斌,罗淑萍,李疆,等. 新疆野巴旦杏天然居群叶片性状表型多样性研究[J]. 新疆农业科学,2008,45(02):221-224.
[16]李利民. 新疆野巴旦杏资源及其开发利用[J]. 新疆农业科学,1997,03:126-128.
[17]吴华清. 梨自交亲和性变异的分子机理研究[D]. 南京:南京农业大学,2006:04.
[18]吴华清,张绍铃,李晓,等.植物自交不亲和性的分子生物学进展[J]. 南京农业大学学报,2006,29(4):119-126.
[19]Lai Z, Wenshi M, Bin H, et al. An F-box gene linked to the self-incompatibility (S) locus of Antirrhinum is expressed specifically in pollen and tapetum[J]. Plant Molecular Biology, 2002, 50(1):29-41.
[20]Ushijima K, Sassa H, Dandekar A M, et al. Structural and Transcriptional Analysis of the Self-Incompatibility Locus of Almond[J]. Plant Cell, 2003,15(3):771-781.
[21]李洪果. 李、杏、杏李S基因型确定及其在种间杂交亲本选择中的应用[D]. 株洲,中南林业科技大学,2010,7
[22]Tetsuyuki E, Megumi I, Hiroshi S, et al. Comparative analysis of the self-incompatibility (S-) locus region of Prunus mume: identification of a pollen-expressed F-box gene with allelic diversity[J]. Genes to Cells, 2003, 8(3):203-213.
[23]Tineke S, Tobutt K R, Vaughan S P, et al. Loss of pollen-S function in two self-compatible selections of Prunus avium is associated with deletion/mutation of an S haplotype-specific F-box gene[J]. Plant Cell, 2005, 17(1):37-51.
[24]Ushijima K, Yamane H, Watari A, et al. The S haplotype-specific F-box protein gene, SFB, is defective in self-compatible haplotypes of Prunus avium and Prunus mume[J]. Plant Journal, 2004, 39(4):573-86.
[25]Hisayo Y, Kazuo I, Koichiro U, et al. A pollen-expressed gene for a novel protein with an F-box motif that is very tightly linked to a gene for S-RNase in two species of cherry, Prunus cerasus and Prunus avium[J]. Plant Cell Physiology, 2003, 44(7):764-769.
[26]Wang Y, Wang X, Mccubbin A G, et al. Genetic mapping and molecular characterization of the self-incompatibility (S) locus in Petunia inflata[J]. Plant Molecular Biology, 2003, 53(4):565-580.
[27]Paja S, Xi W, Skirpan A L, et al. Identification of the pollen determinant of S-RNase-mediated self-incompatibility [J]. Nature, 2004, 429(6989):302-305.