Characterization of Mutation at Locus eIF(iso)4E.a and Development of Related Markers in Chinese Cabbage

doi:10.11924/j.issn.1000-6850.2013-1607

Abstract

Abstract: Interaction between virus of Potyviridae family and eIF4E and eIF(iso)4E is a common mechanism when they infect plant. TuMV of Potyviridae family is the most serious virus resource infecting Chinese cabbage. It can use both eIF4E and eIF(iso)4E when infection and each gene has three copies in genome of Chinese cabbage. Thus it’s necessary to screen the status of each locus among core Chinese cabbage germplasms resistant or susceptible to TuMV. At present study, we characterized three novel haplotypes among five lines possessing intact eIF(iso)4E.a gene; the status of eIF(iso)4E.a pseudogene was identified by Tail-PCR among seven lines studied. A set of codominant markers were developed to distinguish the intact state and pseudogene mutation. The stratage used in the study can still be used to identify status of other loci of eIF4E and eIF(iso)4E family or other functional genes. The markers could be used for breeding new virus-resistant varieties through polymerized cross and marker assisted selection when mutations and markers of other loci were identified.

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