Abstract: The objectives of this study were to improve the frequencies of callus induction and somatic embryogenesis of desirable rubber clones, and optimize the anther plantlets regeneration system. The effects of different concentrations of TDZ on callus induction and somatic embryogenesis were conducted by using anthers of Hevea brasiliensis from 3 clones:‘Yunyan 73-477’,‘Reken 525’and‘Yunyan 77-2’. The results indicated that the optimizing medium for callus induction was MS+TDZ 0.2 mg/L+6-BA 1 mg/L+2,4-D 1.5 mg/ L+NAA 0.5 mg/L+ coconut water 50 mL/L+sucrose 70 g/L. All 3 clones showed highest callus induction rates in this medium, 98%, 98.67% and 28.6% respectively. The induction rate of callus was gradually declining with increasing concentration of TDZ. In the somatic embryogenesis induction test, TDZ restrained the callus regeneration when it existed in callus inducing period. When the callus was then transferred to the subculture medium without TDZ, the induction rate of somatic embryogenesis was rising with the increasing subculture times. And lower TDZ concentration was more favorable for the somatic embryo induction in callus inducing period. The induction rate of somatic embryogenesis was lower and there was no normal embryo when using TDZ instead of KT in the somatic embryogenesis inducing period. These showed that the TDZ was favorable for the callus induction and growth, but not for the somatic embryogenesis induction.