Abstract: This study aimed to comprehensively evaluate different winter green manure on characteristics of nitrogen uptake and utilization, to provide the basis for rational application of N fertilizer and improvement of nitrogen nutrition in high-yield cultivation of rice. This experiment was set to compare milk vetch, rapeseed, ryegrass and mixed of green manure on rice yield, rice growth dynamics and nitrogen uptake and utilization in rice. The results showed that among the 4 different winter green manure, the early rice with treatment of MVR-R (milk vetch- rice-rice) had the highest yield, compared with RG-R-R (ryegrass - rice - rice), RP-R-R (rapeseed- rice - rice), MS-R-R (mixed green manure- rice- rice), treatment by MV-R-R increased rice yield by 6.61%, 3.29%, 0.78%, respectively. The treatment of MV-R-R in N content in plant of rice was maximized in the tillering, booting, heading and maturity periods, higher than the average of the other treatments by 9.68%, 19.72%, 6.23% and 8.66%. At tillering, booting, heading and maturity, the treatment of MV-R-Rwas the highest in N uptake, while RP-R-R was the lowest. The N periodic accumulation for MV-RR were higher than the other treatments in the tillering to booting, booting to heading and heading to maturity periods, with the rate at 21.81%, 68.73% and 286.5% respectively. In addition, N periodic accumulation and its ratio to the total in the heading to maturity was the minimum, the maximum occurred before tillering under green manure rotation system. So the cropping system of milk vetch - rice- rice could increase nitrogen use efficiency and improve N cycling.