Isolation, Identification and Functional Analyses of Endophytic Bacteria from Dendrobium chrysotoxum

doi:10.11924/j.issn.1000-6850.2014-1029

Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (25): 171-176.doi: 10.11924/j.issn.1000-6850.2014-1029

Special Issue: 园艺

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Isolation, Identification and Functional Analyses of Endophytic Bacteria from Dendrobium chrysotoxum

Received:2014-04-10 Revised:2014-05-21 Online:2014-09-05 Published:2014-09-05

Abstract

Abstract: To provide microbial fungicide and biofertilizer production with bacterial sources, endophytic bacteria were isolated from Dendrobium chrysotoxum by tissue culture, antagonistic bacteria were screened by dual culture and inhibitory zone, phosphate- and potassium-solubilizing and nitrogen-fixing bacteria were selected by medium plate, and yield increase by nitrogen-fixing strain was confirmed by field experiments. The bacteria were identified based on their 16S rDNA sequence by PCR amplification, morphology of their cells and colonies, and partial physiological and biochemistry traits. The results showed that 33 strains were obtained with the number order as: root＞stem＞leaf. Among them, GB7 belonged to Bacillus amyloliquefaciens, GB16 to Streptomyces sp., GB8, GB9 and GB21 to Bacillus spp., and all had disease control effect; GB2 was identified as Acinetobacter calcoaceticus, and GB20 as Enterobacter sp., and both had phosphate-solubilizing effect; GB1 was classified into Klebsiella oxytoca with nitrogen-fixing effect.

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Isolation, Identification and Functional Analyses of Endophytic Bacteria from Dendrobium chrysotoxum

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