Abstract: Analysis of genes related to heat stress of eggplant can provide basis for cultivating heat tolerant varieties of eggplant. A SSH-cDNA library of heat-shocked eggplant 896 was constructed by suppression subtractive hybridization method. Real- time quantitative PCR expression analysis further validated the expression modes of partial sequences in this library. Library analysis showed that a large number of unigenes participated in the regulation of eggplant metabolic processes, including carbohydrate, lipid, energy and amino acid metabolic pathways, hormonal regulation of the heat-shocked eggplant expressed as growth inhibition in vivo. YP16 putative encoding a small heat shock protein, YP61 putative encoding carbonic anhydrase and YP248 putative encoding calcium-dependent protein kinase were confirmed to have up-regulated expression in eggplant leaves after the heat-shock.