Abstract: Five different Wuyi Ming Cong tea plant germplasm resources JM054‘( JMH’), JM045‘( JLH’), JM065‘( LLX’), JM024‘( YQL’) and JM028‘( YGY’) were collected. Then the contents of flavonoids, free amino acids, soluble sugar, tea polyphenols and other major biochemical components were measured. Tea polysaccharides (TPS) of each sample were obtained. The contents of protein, neutral sugar, uronic acid, tea polyphenol of each tea polysaccharide were measured. Then Vc was set as a positive control, and the method of D-deoxyribose-iron system were adopted to compare the scavenging activity of hydroxyl radical (·OH) by TPS. The results showed that the contents of soluble sugar in different Wuyi Ming Cong tea plants were significantly different and the free amino acids and tea polyphenols even reached a very significant level, also there was a very significant difference in the contents of uronic acid and neutral sugar in TPS, it illustrated that there was a relatively strong diversity in biochemical composition of Wuyi Ming Cong tea plants and TPS in Wuyi Ming Cong tea plants. There was a significant difference of scavenging rate of hydroxyl radical by TPS in different Wuyi Ming Cong tea plants:‘YGY’-TPS (IC50=4.37 mg/mL)＞‘JMH’-TPS (IC50=4.80 mg/mL)＞‘YQL’- TPS (IC50=6.21 mg/mL)＞‘JLH’-TPS (IC50=6.31 mg/mL)＞‘LLX’-TPS (IC50=6.75 mg/mL), all of them had a better scavenging effect than Vc (IC50=26.03 mg/mL), indicating that TPS in Wuyi Ming Cong tea plants was a kind of good hydroxyl radical scavenger which possesses a strong antioxidant activity, and there was a relatively strong diversity of antioxidant activity of Wuyi Ming Cong tea plants.