Abstract: To determine mineral nutrition and seedling growth of different maize inbred lines under high aluminum (Al) and low phosphorus (P) interaction stress, and understand the relationship between the maize resistance to low P and high Al, a series of pots were used to study the characteristics of growth and uptaking of mineral elements of 8 maize inbred lines at seedling stage under different acid Al and P treatments. The results demonstrated that P deficiency and acid stress severely hindered the growing of maize. There was different tolerance to low-P and acid stress among maize lines. Lines 99320 and 99180 showed tolerance to both low P and acid-aluminum stress. They had higher relative dry matter weight when grew under low P and acid-aluminum stress. Lines 99239 and 99327 showed tolerance to low P but were sensitive to acid-aluminum stress. Line 99003 was tolerant to acid-aluminum stress but sensitive to low P condition. Line 99152 was sensitive to both low P and acid-aluminum stress. The stress of acid-aluminum could cause P deficiency and higher P could alleviate the toxicities. The low P severely affected the absorbing of P, K, Ca, Mg. The stress of acid-aluminum significantly decreased the absorbing of Ca, Mg, P, K and Fe. The addition of P could alleviate the toxicities and result in higher uptake amount of P, K and Fe. We also found that there was no relationship between Al-accumulation of shoot and acid and aluminum tolerance in maize inbred lines.