Effect of Soy Isoflavones on Lactating Performance, hematogenic immunity and antioxidative activity of Dairy Cows

doi:10.11924/j.issn.1000-6850.casb14110164

Abstract

Abstract: The research aimed to study the effect of soy isoflavones on lactating performance, antioxidant capability and immune functions of dairy cows. A single factor design was used, 24 ‘Holstein cows’ were divided into 4 groups randomly. Set group A as the control group, group B, C and D as test groups. Cows in group B, C and D were fed by total mixed ration adding 10, 20, 30 mg/kg soy isoflavones respectively. Milk and blood samples were collected at the 1st, 14th, 21st, 28th, 35th and 42nd days. The results showed that soy isoflavones could significantly enhance milk yield, especially group D whose milk yield increased 21.8% (P<0.05) after 42 days. But no continuable and significant change showed in milk protein rate and butter-fat rate. Lymphocytes and monocytes were significantly reduced but the amount of PMN did not change. The amount of RBC significantly increased (P<0.05) after 28 days while the amount of WBC did not show obviously change. After feeding soy isoflavones for 28 days, the expression of IL-4 in serum enhanced significantly (P<0.05). Soy isoflavones could significantly enhanc the activity of GSH-Px and T-SOD after 35 days (P<0.05). It could be concluded that SI could improve the lactation performance, enhance the immune function and activities of antioxidant enzyme.

References

[1] BAEZA I,NURIA M,DE C,et a1．Improvementof immune cell functions in aged mice treated for five weeks with soybean isoflavones[J]．Annals of the New York Academy of Sciences,2007,11(1)：497—504．
[2]侯建峰,冯笑山,杨晓鹤,等．大豆异黄酮干预对乳腺癌中ERKl、ERK2和c-Fos表达的影响及意义[J]．肿瘤,2010,30(9)：744-747．
[3]陈丰,蒋宗勇,林映才,等．大豆异黄酮对哺乳母猪生产性能及抗氧化性能的影响[J]．饲料博览,2010,(8)：1-5．
[4]蒋守群,蒋宗勇,林映才,等．大豆异黄酮对岭南黄肉鸡生产性能、肉品质和机体抗氧化能力的影响[c]//动物营养与饲料研究：第五届全国饲料营养学术研讨会论文集．珠海：中国畜牧兽医学会动物营养学分会,2006：69—72．
[5]杨建英. 大豆黄酮对不同泌乳期奶牛产奶量、乳成分的影响及相关作用机制的研究[D].河南农业大学,2003.
[6]张少英. 泌乳的激素调控[J]. 国外畜牧学(草食家畜),1990,(1):31-33.
[7]杨建英,张勇法,王艳玲,郭永国,兰尊海. 大豆黄酮对奶牛产奶量和乳中常规成分的影响[J]. 饲料研究,2005,(6):30-31.
[8]程蕾,王定发,刘晓华,王肆玖,凌明湖. 大豆异黄酮对奶牛产奶量及乳成分的影响[J]. 饲料博览,2009,(4):5-7.
[9]郝振荣,朱志宁,郭玉琴,蒋林树,高伟,李艳艳. 大豆黄酮对产奶量和牛奶品质的影响[J]. 北京农学院学报,2009,24(4):26-27,38.
[10]陈杰,杨国宇,韩正康. 大豆黄酮对反刍动物血清睾酮和瘤胃消化代谢的影响[J]. 江苏农业研究,1999,20(2):17-19.
[11]刘春龙,李忠秋,张帆,许岩,姜文博,单安山. 短期人工瘤胃发酵法研究不同水平大豆黄酮和染料木素对奶牛瘤胃液的影响[J]. 动物营养学报,2008,20(3):349-354.
[12]刘春龙,李忠秋,张帆,姜文博,单安山. 大豆黄酮和染料木素对奶牛瘤胃液内主要养分相关酶活性的影响[J]. 中国畜牧杂志,2009,45(5):39-41.
[13]刘兆斌. 大豆黄酮对大鼠肌肉生长、内分泌水平与细胞免疫功能的影响[D].南京农业大学,2003.
[14]刘德义,顾有方,王永鑫,丁春华,陈会良. 大豆黄酮对奶牛血清抗氧化能力和免疫功能的影响[J]. 中国兽医学报,2008,28(11):1306-1309.
[15]许啸,齐智利. 大豆异黄酮对畜禽生理机能的调控[J]. 动物营养学报,2012, 24(3):436-438.
[16]SHUSTER D E, LEE E K, KEHRLI M E Jr. Bacterial growth, inflammatory cytokine production, and neutrophil recruitment during coliform mastitis in cows within ten days after calving, compared with cows at midlactation [J]. American Journal of Veterinary Research, 1996, 57(11):1569 -1575.
[17]PERSSON-WALLKER K,COLDITZ I G,SEOW H F. Accumulation of leucocytes and cytokines in the lactating ovine udder during mastitis due to Staphylcoccusaureus and Escherichia coli [J]. Research inVeterinary Science, 1997, 62(1):63 -66.
[18]朱志宁,郝振荣,王明,蒋林树,郭玉琴. 大豆异黄酮对高产奶牛泌乳后期乳腺肥大细胞分泌肿瘤坏死因子-α和表面型免疫球蛋白A水平的影响[J]. 动物营养学报,2011, 23(1):112-121.
[19] A Ito, B F Bebo, A Matejuk, A Zamora,M Silverman,A Fyfe-Johnson,H Offner. Estrogen treatment down - regulates TNF - alpha production and reduces the severity of experimental autoimmune encephalomyelitis in cytokine knockout mice .[ J ] . Journal of immunology (Baltimore, Md. : 1950), 2001 , 167 ( 1 ) : 542 - 552
[20] Chacko Balu K , Chandler Robert T , Mundhekar Ameya,Khoo Nicholas,Pruitt Heather M,Kucik Dennis F,Parks Dale A , Kevil Christopher G,Barnes Stephen,Patel Rakesh P. Revealing anti - inflammatory mechanisms of soy isoflavones by flow : modulation of leukocyte - endothelial cell interactions [ J ] . American journal of physiology. Heart and circulatory physiology, 2005 , 289 ( 2 ) : H 908 - H 915.
[21] Park Jin, Kim Seung H , Cho Daeho, Kim Tae S. Formononetin , a phyto - oestrogen , and its metabolites up -regulate interleukin - 4 production in activated T cells via increased AP - 1 DNA binding activity [ J ] . Immunology , 2005, 116 ( 1 ) : 71 - 81.
[22]SHIMODA K, VAN DEURSEN J, SANGSTER MY, et al. Lack of IL-4-induced Th2 response and IgE class switching in mice with disruptedStat6 gene [J]. Nature, 1996, 380:630-633.
[23] Han Dalho, Denison Michael Steven, Tachibana Hirofumi, Yamada Koji. Effects of estrogenic compounds on immunoglobulin production by mouse splenocytes [ J ] . Biological pharmaceutical bulletin, 2002, 25 ( 10 ) : 1263 - 1267.
[24]冯敬晶. 日粮中添加大豆异黄酮对大鼠抗热应激能力的影响[J]. 现代农业科学,2009,(6):19-20.
[25]迟晓星,张涛,郑丽娜,袁旭,于开源. 大豆异黄酮对青年雌性大鼠的抗氧化作用研究[J]. 中国食品学报,2010,10(5):78-82.