Abstract: To determine spatial pattern of Sesamia inferens Walker larvae in sugarcane seedling stage, research of sugarcane seedling stage dead heart in YSRI research and demonstration base were conducted in 2012. Spatial pattern of S. inferens larvae was studied by geostatistical methods in different cultivated forms of sugarcane. The results showed that the S. inferens larval density in plant cane (1.07 larvae per site) was significantly lower than that in ratoon cane (1.91 larvae per site), and the variable coefficient, kurtosis and skewness in plant cane were higher than that in ratoon cane. This indicated that the occurrence of the larval population in ratoon cane was more severe than that in plant cane, and the aggregation degree in plant cane was higher than that in ratoon cane. Semivariogram of S. inferens larvae in the four directions in plant cane field could be described by spherical model. Semivariogram of S. inferens larvae in ratoon cane could be described by spherical models in the 45° and 90° directions, Gaussian model in the 0° direction and random distribution in the 135° direction, indicating that larval population showed an overall clumped distribution both in the plant and ratoon cane field, but aggregation degree in plant cane was higher than that in ratoon cane. In the 0° and 45° direction, moderate spatial correlations were found both in plant and ratoon cane, and the correlation distance in ratoon cane was significantly longer than that in plant cane, indicating a stronger spatial correlation in ratoon cane than that in plant cane. The Kriging interpolation diagrams showed that severe occurrence of the larvae was found in the marginal areas in plant cane with a major insect source from the circumjacent cane fields. More and evenly distributed centers could be seen in ratoon cane, suggesting an insect source within the cane field.