Abstract: In order to select a better fertilization method for the no-tillage cultivation of tobacco, the effects of different fertilization methods on yield and quality of tobacco were studied under no-tillage cultivation. The results showed that, no-tillage cultivation and different fertilization methods had no significant effects on the growth of tobacco. At the late growth stage of tobacco, the contents of available nitrogen, available phosphorus, available potassium of row fertilization was higher than that of hole fertilization and row fertilization hole fertilization by 20.6, 16.6, 24.2 mg/kg and 36.4, 17.8, 198.7 mg/kg, respectively, indicating that row fertilization had the better supply capacity of soil nutrient. The yield and output value of row fertilization were higher than that of the hole fertilization and row fertilization hole fertilization by 14.3%, 14.7% and 11.0%, 13.9% respectively, and was higher than that of CK by 7.8% and 14.1%, its average price and high-class-leaf proportion were the highest in all methods. The chemical components of row fertilization were more harmonious in different fertilization methods, the level of quality of sensory evaluation decreased in an order as: row fertilization＞ hole fertilization＞ row fertilization hole fertilization. The row fertilization method can improve the chemical components and the quality of sensory evaluation than other fertilization methods. So, row fertilization of base fertilizer is the best selection of fertilization methods for no-tillage cultivation.