Abstract: In order to analyze the relationship between chlorine content and organic acids content and sensory quality, 294 samples of flue-cured tobacco leaves from main tobacco-growing areas of China were collected f or analyzing the quantitative characteristics of chlorine and organic acid content of flue- cured tobacco by descriptive statistics, and studying changes of organic acids content and sensory quality at different levels of chlorine content in flue-cured tobacco by clustering method. The results indicated that chlorine content in flue-cured tobacco leaves ranged from 0.08% to 1.60%, with the mean of 0.44% and standard deviation of 0.28% . There were significant differences among different tobacco growing- areas at 5% level, but no significant differences were found among different stalk positions. On the basis of chlorine content, samples were divided into low, medium and high groups by cluster analysis: the group with low chlorine content had the lowest contents of non-volatile organic acid and total organic acids, but medium contents of senior fatty acid and volatile organic acid; the group with medium chlorine content had the highest contents of organic acids; the group with the highest chlorine content had medium contents of non-volatile organic acid and total organic acids, but had the lowest contents of senior fatty acid and volatile organic acid. There existed significant negative correlation between sensory quality and chlorine-content of flue-cured tobacco; the total smoking score gradually decreased with the increase of chlorine-content.