Study on Physiological Injury of Watermelon Leaves Under Boron Stress by Histological Stain Method

doi:10.11924/j.issn.1000-6850.casb15080134

Abstract

Abstract: In order to study the physiological injury to watermelon leaves caused by boron stress, seed-used watermelon, watermelon cultivar‘Heimeiren’and wild watermelon were used to observe the activities of POD, the content of ROC (O2·? and H2O2) and dead cells under 4 boron concentrations by histological stain method. The results showed that there were lots of ROC and dead cells, the activities of POD increased in watermelon leaves under boron stress. The high boron stress led to more ROC and dead cells in the leaves of the three watermelon cultivars. The scavenging reactive oxygen species of wild watermelon was stronger than that of the other two watermelon cultivars. Boron stress led to the active oxygen accumulation in watermelon leaves, the activities of antioxidant enzymes increased and a large number of cells died. The physiological injury to watermelon leaves caused by high boron stress was more serious than that caused by boron deficiency. Stress resistance of wild watermelon was the strongest. Histological stain method is accurate, intuitive, and can be used in the stress physiology research on plants such as watermelon.

References

[1] Raven J A．Short-and Long-Distance Transport of Boric Acid in Plants[J]．New Phytologist,1980,84(2)：231-249．
[2] 黄益宗．植物对硼素不足的反应及其成因探讨[J]．土壤与环境,2002,11(4)：434-438．
[3] 徐照丽,杨宇虹,段玉琪,等．硼肥对不同烤烟品种生长发育及产质量的影响[J]．华北农学报,2013(S1)：342-346．
[4] 王瑞东,姜存仓,刘桂东,等．常规与缓释硼肥连施及停施对脐橙硼营养的影响[J]．中国南方果树,2014,43(2)：41-43．
[5] 曹秀霞,安维太,万海霞．硼肥对旱地胡麻生长及产量的影响[J]．北方园艺,2015(6)：154-157．
[6] 龙友华,张承,吴小毛,等．叶面喷施硼肥对猕猴桃产量及品质的影响[J]．北方园艺,2015(5)：9-12．
[7] 刘鹏．硼胁迫对植物的影响及硼与其它元素关系的研究进展[J]．农业环境保护,2002,21(4)：372-374．
[8] 杨晓玲．硼钼组合对盐藻生长与物质积累的作用[J]．江苏农业科学,2013,41(11)：265-266．
[9] 石艳星,刘树庆．硼酸／双氰胺双抑制剂缓释肥对油菜产量及氮素利用率影响[J]．北方园艺,2014(19)：183-187．
[10] 南丽丽,师尚礼,陈建纲,等．硼锌配施对苜蓿矿质元素和碳水化合物含量的影响[J]．中国草地学报,2013(1)：23-28．
[11] 耿明建,朱建华,吴礼树,等．硼对不同硼效率棉花品种苗期叶片膜脂过氧化和多胺含量的影响[J]．植物营养与肥料学报,2003,9(003)：337-341．
[12] 刘鹏,杨玉爱．钼,硼对大豆叶片膜脂过氧化及体内保护系统的影响[J]．植物学报：英文版,2000,42(5)：461-466．
[13] 杨暹,陈晓燕．硼钼营养对青花菜花球产量及活性氧代谢的影响[J]．园艺学报,2000,27(2)：112-116．
[14] 梁诗．硼对大白菜生长发育及若干生理生化指标的影响[J]．亚热带植物科学,2002,31(3)：19-22．
[15] 李晓慧,张恩让．亚高温及外源物质调节下番茄的生理响应[J]．江苏农业科学,2013,41(7)：135-137．
[16] Doke N,Ohashi Y．Involvement of an O₂— generating system in the induction of necrotic lesions on tobacco leaves infected with tobacco mosaic virus[J]．Physiological and molecular plant pathology,1988,32(1)：163-175．
[17] Schraudner M,Moeder W,Wiese C,et al．Ozone-induced oxidative burst in the ozone biomonitor plant, tobacco Bel W 3[J]．Plant Physiol,1998,16(2)：235-245．
[18] Thordal-Christensen H,Zhang Z,Wei Y,et al．Subcellular localization of H₂O₂ in plants. H₂O₂ accumulation in papillae and hypersensitive response during the barleypowdery mildew interaction[J]．The Plant Journal,1997,11(6)：1187-1194．
[19] McLusky S R,Bennett M H,Beale M H,et al．Cell wall alterations and localized accumulation of feruloyl-3'-methoxytyramine in onion epidermis at sites of attempted penetration by Botrytis allii are associated with actin polarisation, peroxidase activity and suppression of flavonoid biosynthesis[J]．The Plant Journal,1999,17(5)：523-534．
[20] Vranova E,Inze D,Van Breusegem F．Signal transduction during oxidative stress[J]．Journal of Experimental Botany,2002,53(372)：1227．
[21] Dunford H B,Stillman J S．On the function and mechanism of action of peroxidases[J]．Coordination Chemistry Reviews,1976,19(3)：187-251．
[22] McCann M C,Shi J,Roberts K,et al．Changes in pectin structure and localization during the growth of unadapted and NaCl-adapted tobacco cells[J]．Plant J,1994,5(6)：773-785．
[23] Cakmak I,Romhekd V．Boron deficiency-induced impairments of cellular functions in plants[J]．Plant and soil,1997,193(1)：71-83．
[24] 施木田,陈如凯．锌硼营养对苦瓜产量品质与叶片多胺、激素及衰老的影响[J]．应用生态学报,2004,15(1)：77-80．
[25] 陆旺金,沈康,沈振国．硼素胁迫影响油菜₁₄CO₂同化及其产物运输的研究[J]．作物研究,1992,6(2)：33-36．
[26] 王春利．植物缺硼的生理伤害[J]．植物生理学通讯,2001,37(4)：352-355．
[27] Netsangtip R,Bell R W,Dell B,et al．An effect of light on the B requirement for leaf blade elongation in black gram (Vigna mungo)[J]．Plant and Soil ,1993,155(156)：413-416．
[28] Appel H M．Phenolics in ecological interactions: The importance of oxidation[J]．J Chem Ecol,1993,19(7)：1521-1552．
[29] Dordas C,H.Brown P．Boron deficiency affects cell viability, phenolic leakage and oxidative burst in rose cell cultures[J]．Plant and soil ,2005,268(1)：293-301．