Strawberry Plantlets Somaclonal Variation

doi:10.11924/j.issn.1000-6850.casb15100124

Abstract

Abstract: Variations are easily induced in tissue culture of plants. They significantly affect the genetic stability of plants, and limit the application of tissue culture technology in agriculture. Strawberry in vitro seedlings have been widely used in agricultural production. However, the variations often occur in tissue culture of plant, which seriously influence the agricultural production efficiency. Therefore, it is an urgent problem to effectively control the occurrence of the variations in vitro rapid propagation of strawberry and other plants. The main factors affecting the occurrence of the variations were analyzed. These factors included explant types, genotypes, the types and proportion of different hormones in the medium, the time and times of subculture. And the inherent genetic mechanism of the variations had chromosomal abnormalities, transposon activation, gene mutation and so on. In order to effectively control the variation occurrence in tissue culture of strawberry, it was suggested to select the young explants, optimize the culture medium formula, control the multiplication coefficient and the subculture times, and culture the tissues under proper light and temperature conditions. Meanwhile, using the methods of morphological observation, RAPD, SSR and so on to detect the variations in tissue culture seedlings could eliminate the variant seedlings and reduce the adverse effects of the variant plants to production. By summarizing the causes and the effective controlling ways of the variation of strawberry in vitro, it would provide valuable reference for the healthy development of plant virus-free tissue and rapid propagation of plants including strawberry. Moreover, the deep understanding of variation would provide a new way for the improvement and breeding of new varieties.

CLC Number:

S668.4

Hu Panpan,Zhao Xia,Li Gang,Li Liangjie,Qin Bao,Peng Zhuo and Zhou Houcheng. Strawberry Plantlets Somaclonal Variation[J]. Chinese Agricultural Science Bulletin, 2016, 32(16): 77-82.

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