Identification of Maize Hybrid Varieties Using SSR Marker

doi:10.11924/j.issn.1000-6850.casb15110025

Abstract

Abstract: Purity is an important parameter of seed quality. ‘Jixiang No 1’ is a main maize hybrid variety and widely cultivated in Gansu Province. To identify the purity of the variety, the authors conducted a research on SSR purity identification method of ‘Jixiang No 1’. Through the comparison of rapid extraction method and improved CTAB method used in the purity test of ‘Jixiang No 1’ with SSR marker, the results showed that the DNA extracted by the above two methods was applicable to the purity detection of SSR molecular markers, but the rapid extraction method had the characteristics of simple, fast, and was more suitable for the purity identification. bnlg2291k4 and bnlg2305k4 were selected from 20 pairs of SSR markers for purity test of ‘Jixiang No 1’, both markers could clearly distinguish parents and hybrids. Mixing parents seeds into hybrid seeds of ‘Jixiang No 1’, the method established by this study could clearly identify inbred lines.

Che Zhuo,Chang Hong, and Zhang Wei. Identification of Maize Hybrid Varieties Using SSR Marker[J]. Chinese Agricultural Science Bulletin, 2016, 32(18): 28-32.

References

[1] Crow J F. 90 years ago: The beginning of hybrid maize[J]. Genetics,1998,148:923-928.
[2] 石德权,郭庆法.中国玉米品质现状、问题及发展优质食用玉米对策[J].玉米科学,2001,9(2):3-7.
[3] 施骥,李宁.中国玉米育种发展现状及发展方向探讨[J].农业科技通讯,2012(5):5-7.
[4] 孔菲.SSR分子标记在玉米种子纯度鉴定中的应用[J].种子世界, 2011(2):33-34.
[5] Smith J S C, Register M J C. Genetic purity and testing technologies for seed quality: A company perspective[ J] . Seed Science Reseach,1998,8:285-293.
[6] Li X, Yuan L, Li M. Methods and techniques of variety purity test in maize[J]. Theory and Application of Crop Research, 2000: 56-63.
[7] Chin E, Senior L, Shu H, et al. Maize simple repetitive DNA sequence: Abundance and allele variation. Genome,1996,39:866- 873.
[8] 王懿波,王振华.中国玉米主要种质的改良与杂优模式的利用[J].玉米科学,1999,7(1):1-8.
[9] McDonald M B, Elliot L J, Sweeney P M. DNA extraction from dry seeds for RAPD analysis in varietal identification studies.Seed Sci. & Tech.1994,22:171-176.
[10] Smith J, Smith O. Retriction Fragment Length Polymorphisms Can Differentiate among US Maize Hybrids[J]. Crop science,1991,31(4):893-899.
[11] 辛景树,郭景伦,贾希海. SSR 技术在玉米种子纯度鉴定上的应用(上)[J].种子科技,2005,23(3):155-157.
[12] Perry D J. Identification of Canadian durum wheat varieties using a single PCR[J]. Theor ApplGenet,2004,109:55-61.
[13] McDonald M B. Genetic purity: From protein electrophoresis to RAPDs[A]. Ann. Proc. of Corn and Sorghum Res. Conf, .Amer. Seed Trade Assoc.[C], Washington, D.C,1996,50:256- 271.
[14] 曹士亮,曹靖生,王成波,等.玉米SSR分子标记技术操作流程研究进展[J].中国农学通报,2012(15):1-4.
[15] 戴剑,洪德林. SSR分子标记技术在杂交水稻品种鉴定中的应用[J].金陵科技学院学报,2012,28(1):45-51.
[16] 郭奕生,何德银.SSR分子标记在玉米品种真实性鉴定上的应用[J].广东农业科学,2014(12):12-15.
[17] 郎需勇,吴建功,杨亮,等. SSR分子标记技术在杂交棉纯度鉴定中的应用[J].中国棉花,2012(9):17-23.
[18] 赵欣欣,张新全,苗佳敏,等.多花黑麦草杂交种SSR分子标记鉴定及表型比较分析[J].农业生物技术学报,2013,21(7):811-819.
[19] Smith J S C, Chin E C L, Shu H, et al. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.):Comparison with data from RFLPs and pedigree[J]. Theoretical and Applied Genetics,1997(95):163-173.
[20] 魏臻武.利用SSR、ISSR和RAPD技术构建苜蓿基因组DNA指纹图谱[J].草业学报,2004,13(3):62-67.
[21] 严学兵,王堃,周禾,等.不同来源SSR标记在中国披碱草属植物的通用性和效率评价[J].草业学报,2008,17(6):112-120.
[22] 郭海林,刘建秀,高鹤,等.结缕草属优良品系SSR指纹图谱的构建[J].草业学报,2007,16(2):53-59.
[23] Weising K, Nybom H, Pfenninger M, et al. DNA Fingerprinting in Plants and Fungi : Principles, Methods, and Applications[M]. Boca Raton, FL::CRC Press,1994.
[24] 郭景伦,赵久然,王凤格.适用于SSR分子标记的玉米单粒种子DNA快速提取新方法[J].玉米科学,2005,13(2):16-17.
[25] Virk P G, Pooni H S, Syed N H, et al. Fast and reliable geno-type validation using microsatellite markers in Arabidopsis thaliana[J].Theor ApplGenet,1999,98:462-464.
[26] Paris M, Carter M. Cereal DNA: Arapid high-throughput extrac-tion method for marker assisted selection[J]. Plant Molecular Biology Reporter: JoumalofMaize Sciences,2000,18:357-360.
[27] Baker T K, M acck J J, Metz S G, et al. Chemical Control Of Pollination for hybrid Wheat Production [J]. Crop science,1995(35):55-55.
[28] Holden M J, Blasic J R, Bussjaeger L, et al. Evaluation of extraction methodologies for corn kernel (Zea mays) DNA for de?tection of trace amounts of biotechnology derived DNA[J]. Journal of Agricultural and Food Chemistry,2003,51(9):2468-2474.
[29] Peano C, Samson M C, Palmieri L, et al. Qualitative and quantitative evaluation of the genomic DNA extracted from GMOand non?GMO foodstuffs with four different extraction methods[ J]. Journal of Agricultural and Food Chemistry, 2004,52(23): 6962-6968.
[30] Di Bernardo G, Del Gaudio S, Galderisi U, et al. Comparative evaluation of different DNA extraction procedures from foodsamples[J]. Biotechnol Prog,2007,23(2):297-301.
[31] 朱志成. SSR分子标记技术在玉米杂交种纯度鉴定上的应用[J].河南农业,2011(5):52.
[32] 黄成志,雷树凡,胡景涛,等.利用优化的SSR分子标记技术检测杂交水稻万优481纯度[J].安徽农业科学,2014,42(22):7321-7322.
[33] 郭承亮. SSR分子标记在杂交稻种子纯度精确鉴定中的应用[J].中国种业,2014(1):21-23.
[34] 郭承亮,王世才. SSR分子标记在杂交稻种子纯度及杂株类型定量鉴定中的应用[J].中国农技推广,2013(S1):108-111.
[35] 沈童伟,陆徐忠,刘勋辉,等. SSR 标记鉴定玉米品种真实性及纯度的研究[J].安徽农业科学,2009(19):8904-8908.
[36] 马志强,陈洁,郑根昌,等.分子标记方法鉴定玉米杂交种哲单20纯度的研究[J].内蒙古民族大学学报,2007,22(3):293-296.