Abstract: In order to reducing the occurrence and damage of cowpea anthracnose, decreasing the chemical pesticide consumption, residue and environmental pollution, the methods of forecasting, randomized block design and statistical analysis were used to the field efficacy trials, which conducted of the control effect of cowpea anthracnose by using bio-pesticide : Chitosan,biochemical compound preparation Prochloraz-Chitosan,chemical pesticides Difenoconazole and Thifluzamide. The results showed that after applying 2% Chitosan AS 2250g,46%Prochloraz-Chitosan EW 450g,10%Difenoconazole WG900g(Control agent)and 24%Thifluzamide SC360mL per hm₂ per 5-8 days period, and continuous applied 3 times during serious morbidity autumn in greenhouse, the control effects were all over 83%, 78% and 73%, respectively, at 7 days, 14 days and 20 days after treatment.The control effects between 4 kinds of agents were no significant difference. These results indicated the 4 kinds of agents, especially that Chitosan and Prochloraz-Chitosan were ideal bio-pesticides, which could not only be used for controlling tomato virus diseases, but also realize modern, pollution-free, green and organic agricultural production.