Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (18): 160-164.doi: 10.11924/j.issn.1000-6850.casb16020023

Special Issue: 烟草种植与生产

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Inhibitory Effect of Plant Extracts on TMV

Lv Haoqiu1, Wu Junlin1, Wang Cuican1, Li Weihua1, Li Xiang1, He Ping1, Yu Zonghan1, Ma Chao2   

  1. (1Tobacco Corporation of Zhumadian, Biyang Branch, Biyang Henan 463700;2College of Agriculture, Henan University of Science and Technology, Luoyang Henan 471003)
  • Received:2016-02-03 Revised:2016-06-11 Accepted:2016-02-19 Online:2016-06-28 Published:2016-06-28

Abstract: The study aims to identify the inhibitory effect of pokeweed and gynostemma plant extracts on TMV, explore a new method to control TMV diseases, and provide a theoretical basis for the development of plant extracts against TMV. Half leaf method and field spraying were taken as experimental methods, the inhibitory effect of pokeweed and gynostemma plant extracts on TMV and TMV rate were studied. The results of half leaf method showed that chemical antivirus agents all exhibited good inhibitory effects against TMV at 25-200 μg/mL with the inhibition ratios all above 80%. The inhibition ratios after different mixing times with TMV were above 90%. The inhibitory effects of pokeweed and gynostemma crude plant extracts showed that methanol extract > benzene extract > petroleum ether extract, and pokeweed showed a better inhibition ratio in comparing with gynostemma. When pokeweed was mixed with TMV under the concentration of 200 μg/mL, the inhibition ratio increased with the increase of mixing time. The inhibitory effect on field tobacco was compounded preparation > pokeweed > gynostemma, and high concentration of extracts showed better effect than that of low concentration. Agronomic traits of infected tobacco were alleviated by different plant extracts. Pokeweed and gynostemma had obvious inhibitory effects on TMV and could be ideal plant sources to control TMV.

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