Genetic Diversity and Clustering Analysis of Blueberry Resources by CDDP Markers

doi:10.11924/j.issn.1000-6850.casb16020078

Abstract

Abstract: For evaluating and utilizing blueberry resources scientifically and effectively, the genetic diversity among 32 blueberry resources by conserved DNA-derived polymorphism (CDDP) markers and the correlation between CDDP markers and biological characters of blueberry were analyzed in this study. CDDP primers were screened according to their polymorphism and legibility. DNA samples of 32 blueberry resources were amplified by CDDP primers. The genetic diversity and relationship among resources were analyzed based on the statistical result. Using selected 17 informative and reliable primers, 188 (90.82%) polymorphic and 17(8.21%) specific bands were detected out of 207 bands among 32 blueberry accessions. At population level, the effective number of alleles, Nei’s gene diversity (H) and Shannon’s information index (I) was 1.59, 0.37 and 0.56, respectively. All 32 blueberry accessions could be distinguished with 2 primers combination. The SM similarity coefficient among different genotypes varied from 0.44 to 1.00 with an average of 0.73. The dendrogram constructed by UPMGA method showed that all the accessions could be divided into three major groups at similarity coefficient of 0.69, Vaccinium uliginosum L. was separated from other cultivars and clustered to one group, low bush cultivar ‘Blomidon’ and half high bush cultivar ‘Northblue’ were clustered into one group, and the other 29 cultivars clustered into one group. CDDP markers could effectively reveal the genetic diversity of blueberry resources. It was shown that the clustering partly corresponded to cold resistance, chilling requirement and fruit color of blueberry resources. The results demonstrate that CDDP marker is a very effective and efficient tool for genetic diversity analysis, cultivar identification and molecular mark assisted breeding.

CLC Number:

S330

Fang Wenxiu,Xia Xiuying,An Lijia and Peng Qiang. Genetic Diversity and Clustering Analysis of Blueberry Resources by CDDP Markers[J]. Chinese Agricultural Science Bulletin, 2016, 32(28): 136-143.

References

[1] Prior R L, Cao G, Martin A, et al. Antioxidant capacity as influenced by total phenolic and anthocyanin content, maturity and variety of Vaccinium species[J]. Journal of Agricultural and Food Chemistry,1998,46:2586-2593.
[2] Basu A, Du M, Leyva M J, et al. Blueberries decrease cardiovascular risk factors in obese men and women with metabolic syndrome[J]. Journal of Nutrition,2010,140(9):1582-1587.
[3] Adams L S, Phung S, Yee N, et al. Blueberry phytochemicals inhibit growth and metastatic potential of MDA-MB-231 breast cancer cells through modulation of the phosphatidylinositol 3-kinase pathway[J]. Cancer Research,2010,70(9):3594-3605.
[4] Xia X Y, Xu N, Xu D K, et al. Identifiction of the varieties and analysis on genetic relationship of the wild and cultivated species of Vaccinium spp. in China using RAPD markers[J]. Korean Journal of Genetic,2008,30(1):17-22.
[5] 於虹,顾姻,贺善安,等.越橘品种的ISSR鉴定及其亲缘关系分析[J].吉林农业大学学报,2009,31(5):512-515.
[6] 宋杨,张红军,窦连登,等.越橘品种资源亲缘关系的ISSR分析[J].中国果树,2014(6):17-20.
[7] 郑姗,张立杰,谢丽雪,等.蓝莓品种ISSR指纹图谱构建的初步研究[J].福建农业学报,2014,29(12):1198-1201.
[8] 崔建民,刘红霞,邹荣仟,等.越橘种质资源遗传多样性和亲缘关系研究[J].果树学报,2010,27(3):373-378.
[9] 郭照东,夏秀英,安利佳,等.基于SSR标记的越橘亲缘关系分析及品种鉴定[J].植物遗传资源学报,2015,16(5):1020-1026.
[10] Alok R S, Ramya R M, Sobha C R, et al. Construction of interspecific genetic linkage map of pigeonpea using SCoT, RAPD, ISSR markers and simple inherited trait loci[J]. Nucleus,2015,58(1):23-31.
[11] Seda N, Hilal B K, Bahattin T. Genetic assessment of common bean (Phaseolus vulgaris L.) accessions by peroxidase gene-based markers[J]. Journal of the Science of Food and Agriculture,2014,94:1672-1680.
[12] Collard B C Y, Mackill D J. Conserved DNA-derived polymorphism (CDDP): a simple and novel method for generating DNA markers in plants[J]. Plant Molecular Biology Reporter,2009,27:558-562.
[13] Poczai P, Varga I, Laos M, et al. Advances in plant gene-targeted and functional markers: a review[J]. Plant Methods,2013,9:6.
[14] Poczai P, Varga I, Bell N E, et al. Genetic diversity assessment of bittersweet (Solanum dulcamara, Solanaceae) germplasm using conserved DNA-derived polymorphism and intron-targeting markers[J]. Annals of Applied Biology,2011,159:141-153.
[15] Wang X W, Fan H M, Li Y Y, et al. Analysis of genetic relationships in tree peony of different colors using conserved DNA-derived polymorphism markers[J]. Scientia Horticulturae,2014,175:68-73.
[16] 李莹莹,郑成淑.利用CDDP标记的菏泽牡丹品种资源的遗传多样性[J].中国农业科学,2013,46(13):2739-2750.
[17] Li T, Guo J E, Li Y Y, et al. Genetic diversity assessment of chrysanthemum germplasm using conserved DNA-derived polymorphism markers[J]. Scientia Horticulturae,2013,162:271-277.
[18] 李田,郑成淑.菊花品种的遗传多样性分析及CDDP指纹图谱构建[J].北京林业大学学报,2014,36(4):94-101.
[19] 李强,苏二虎,高聚林,等.大豆CDDP分子标记技术体系的优化、验证及引物筛选[J].大豆科学,2013,32(3):310-320.
[20] Cummins’ N J. Register of new fruit and nut varieties list 35[J]. Horticulture Science,1991,26(8):951-986.
[21] Okie W R. Register of new fruit and nut varieties list 39[J]. Horticulture Science,1999,34(2):181-205.
[22] Clark R J. Finn E C. Register of new fruit and nut varieties list 43[J]. Horticulture Science,2006,41(5):1101-1133.
[23] Lyrene P M. Value of various taxa in breeding tetraploid blueberries in Florida[J]. Euphytica,1997,94:15-22.
[24] Zhao X, Zhan L P, Zou X Z. Improvement of cold tolerance of the half-high bush Northland blueberry by transformation with the LEA gene from Tamarix androssowii[J]. Plant Growth Regulation,2011,63:13-22.
[25] Bian Y, Ballington J, Raja A, et al. Patterns of simple sequence repeats in cultivated blueberries (Vaccinium section Cyanococcus spp.) and their use in revealing genetic diversity and population structure[J]. Molecular Breeding, 2014, 34: 675-689.
[26] Inostroza-Blancheteau C, Aquea F, Reyes- D?′az M, et al. Identification of aluminum-regulated genes by cDNA-AFLP analysis of roots in two contrasting genotypes of highbush blueberry (Vaccinium corymbosum L.)[J]. Molecular Biotechnology,2011,49:32-41.
[27] 徐国辉,王贺新,高雄梅.近十年美国蓝莓新品种资源及其特征[J].中国南方果树,2015,44(4):138-144.
[28] Lyrene P M, Vorsa N, Ballington J R. Polyploidy and sexual polyploidization in the genus Vaccinium[J]. Euphytica,2003,133:27-36.
[29] Rowland J L, Ogden L E, Bassil N, et al. Construction of a genetic linkage map of an interspeci?c diploid blueberry population and identi?cation of QTL for chilling requirement and cold hardiness[J]. Molecular Breeding,2014,34:2033-2048.
[30] 孙海悦,李亚东.世界蓝莓育种概述[J].东北农业大学学报,2014,45(9):116-122.
[31] 李宪平.野生笃斯越橘驯化栽培研究:[D].北京:中国农业科学院,2007:4-5.
[32] 尹德洁.蓝莓野生资源和SRAP遗传多样性研究:[D].北京:北京林业大学,2012:44-46.
[33] Giongo L, Vendramin E, Quarta R, et al. Use of RAPD analysis to characterize Vaccinium accessions[J]. Acta Horticulturae,2006,715:154-156.
[34] Garriga M, Parra P A, Caligari P D S, et al. Application of inter simple sequence repeats relative to simple sequence repeats as a molecular marker system for indexing blueberry cultivars[J]. Canadian Journal of Plant Science,2013,93(5):913-921.
[35] 张鲁杰,郭照东,房文秀,等.蓝莓品种的SRAP遗传多样性分析及品种鉴定[J].分子植物育种,2015,13(12):2794-2802.
[36] 王小文.利用CDDP分析不同颜色牡丹的遗传关系[J].中国观赏园艺研究进展,2014:65-73.