Abstract: In order to study the yield traits of hybrid rice under different nitrogen levels, four high-yield hybrid rice varieties (‘Yixiangyou 2115’, ‘Gangyou 188’, ‘Yixiangyou 1577’, ‘Gangyou 1577’) which were widely applied were used as materials in the nitrogen level experiments. The results showed that yield traits were regulated by rice varieties and nitrogen levels. The yields of ‘Yixiangyou 2115’ were not significantly different under different nitrogen levels. With the increase of nitrogen level, the yield of ‘Gangyou 188’ increased significantly. ‘Yixiangyou 1577’ and ‘Gangyou 1577’ exhibited the highest yield under normal nitrogen level. The order of the effects of the yield traits on yield under different nitrogen levels was grain number of per spike＞ panicle length＞ seed set percentage＞ effective panicle, and the effect of grain number of per spike was very remarkable. Therefore, there were significant differences in the sensitivity of different hybrid rice varieties to nitrogen. In production, the optimal hybrid rice variety needs to be selected according to the field fertility level.