Abstract: This experiment select mushroom K5 as experimental material and sets four kinds of cultivation medium including hardwood,mulberry trees,apple trees and jujube trees,and each medium set three treatment levels of nitrogen content.We attempt to study the influence of different cultivation medium to the yield and nutrient composition of the first tide mushroom through testing and analyzing the yield and the content of organic matter,nitrogen,phosphorus and potassium.The result indicates that four kinds of fruit trees can be used as the cultivation medium of mushroom, and the production and the biological efficiency of the jujube C and mulberry A are higher than others.But when the mulberry trees as the cultivation medium,the mushroom-stick began to shrink after the first tide,and it is not conducive to the late fruiting.The nutritional including the organic matter,nitrogen,phosphorus,potassium of the jujube C are at a higher level,therefore,jujube trees can be used instead of the traditional hardwood as the cultivation medium in the mushroom substitute cultivation.