Research Progress of Protoplast Culture in Cereal Crops

doi:10.11924/j.issn.1000-6850.casb16060055

Abstract

Abstract: Plant protoplast has important research value in the application research of the variety improvement, the genetic transformation and the distant hybridization, and in the basic theoretical study of the biology. In order to provide the theoretical basis for the establishment of the system of protoplast culture and promote the work of the genetic improvement of cereal crops varieties, a detailed elaboration about the study on protoplast culture of cereal crops from four aspects such as protoplast preparation, culture, regeneration and application was made in the article. The main factors affecting the protoplast separation and culture were analyzed, the three ways of protoplast regeneration were summed up, and its applications in genetic improvement and theoretical study were summarized. At the same time, the problems in three aspects of the protoplast culture, the genetic transformation and the cell fusion were put forward and future research efforts were also proposed.

CLC Number:

S188

References

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