Bioactive Peptides Based on Plants: A Review

doi:10.11924/j.issn.1000-6850.casb2021-0630

Abstract

Abstract:

The aim is to promote the development and utilization of more bioactive peptides based on plants. The advance in extraction, preparation, isolation, identification and function verification of bioactive peptides were summarized, and the effects of different research methods and their application were compared and analyzed. Then, main problems in current research on bioactive peptides based on plants were summarized. At last, the study proposed that we should reduce the extraction cost and strengthen the standard system construction, which could lay a foundation for the future research of bioactive peptides based on plants.

Key words: plant bioactive peptides, polypeptide preparation, polypeptide isolation, polypeptide function, function verification

CLC Number:

S188

ZHANG Zuoda, WU Ruona, WANG Qinfei, NIU Xiaolei, ZHANG Zhenwen. Bioactive Peptides Based on Plants: A Review[J]. Chinese Agricultural Science Bulletin, 2022, 38(12): 88-94.

Figures/Tables 6

References 60

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标准名称	标准号
进出口动物源性食品中多肽类兽药残留量的测定液相色谱-质谱/质谱法	SN/T 2748-2010
生物活性肽功效评价	GB/T 38790.1-2020
多肽抗氧化性测定 DPPH和ABTS法	GB/T 39100-2020
多肽抗菌性测定抑菌圈法	GB/T 39101-2020
食品安全国家标准胶原蛋白肽	GB 31645-2018
食品安全国家标准食品营养强化剂酪蛋白磷酸肽	GB 31617-2014
大豆肽粉	GB/T 22492-2008
玉米低聚肽粉	QB/T 4707-2014
小麦低聚肽粉	QB/T 5298-2018
饲料中杆菌肽锌的测定高效液相色谱法	NY/T 726-2003
进出口食用动物、饲料中杆菌肽的检测方法	SN/T 4807-2017
饲料中那西肽的测定高效液相色谱法	NY/T 3480-2019
食品营养强化剂酪蛋白磷酸肽	T/ZZB 1826-2020
特级大豆蛋白肽	T/CCOA 28-2020
抗菌肽活性检测技术规程	T/GDP 021-2020
叶枯肽检测方法	JAP-158

标准名称	标准号
进出口动物源性食品中多肽类兽药残留量的测定液相色谱-质谱/质谱法	SN/T 2748-2010
生物活性肽功效评价	GB/T 38790.1-2020
多肽抗氧化性测定 DPPH和ABTS法	GB/T 39100-2020
多肽抗菌性测定抑菌圈法	GB/T 39101-2020
食品安全国家标准胶原蛋白肽	GB 31645-2018
食品安全国家标准食品营养强化剂酪蛋白磷酸肽	GB 31617-2014
大豆肽粉	GB/T 22492-2008
玉米低聚肽粉	QB/T 4707-2014
小麦低聚肽粉	QB/T 5298-2018
饲料中杆菌肽锌的测定高效液相色谱法	NY/T 726-2003
进出口食用动物、饲料中杆菌肽的检测方法	SN/T 4807-2017
饲料中那西肽的测定高效液相色谱法	NY/T 3480-2019
食品营养强化剂酪蛋白磷酸肽	T/ZZB 1826-2020
特级大豆蛋白肽	T/CCOA 28-2020
抗菌肽活性检测技术规程	T/GDP 021-2020
叶枯肽检测方法	JAP-158

酶解对象	种类	酶活力/(U/g)	最适温度/℃	最适pH值	水解时间/h
葵花籽^[9]	木瓜蛋白酶	27	60.0	6.5	2
谷朊粉^[10]	胰蛋白酶	4056	48.0	11.0	4
核桃^[11]	胃蛋白酶	25	35.0	3.0	3
芡实^[12]	中性蛋白酶	3000	45.0	8.0	3
芡实^[12]	碱性蛋白酶	4000	50.0	9.5	3

酶解对象	种类	酶活力/(U/g)	最适温度/℃	最适pH值	水解时间/h
葵花籽^[9]	木瓜蛋白酶	27	60.0	6.5	2
谷朊粉^[10]	胰蛋白酶	4056	48.0	11.0	4
核桃^[11]	胃蛋白酶	25	35.0	3.0	3
芡实^[12]	中性蛋白酶	3000	45.0	8.0	3
芡实^[12]	碱性蛋白酶	4000	50.0	9.5	3

分离膜	膜类型	孔径大小/nm	驱动力	主要应用
微滤	对称微孔膜	50~10000.0	0.05~0.5 Mpa	除菌、澄清、颗粒除杂
超滤	不对称微孔膜	2~50.0	0.2~1.0 Mpa	除菌、澄清、浓缩分离
透析	对称、不对称膜	0.2~14.0	浓度差	小分子、无机盐分离
纳滤	对称微孔膜	小于2.0	0.35-3.0 Mpa	分离、除盐、脱色、浓缩
反渗透	不对称复合膜	小于0.5	1.0-10.0Mpa	浓缩、纯化、海水淡化
渗透汽化	致密或复合膜	小于0.5	浓度差	有机溶剂脱水、纯化