Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2017, Vol. 33 ›› Issue (5): 95-100.doi: 10.11924/j.issn.1000-6850.casb16060124

Special Issue: 生物技术 畜牧兽医

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Optimization of Bovine Somatic Cell Nuclear Transfer Technology System

  

  • Received:2016-06-24 Revised:2017-01-06 Accepted:2016-09-20 Online:2017-03-01 Published:2017-03-01

Abstract: To improve the quality of oocyte maturation and developmental competence of nuclear transfer embryos, different concentrations of IGF and FBS were added into oocyte mature medium and embryo nutrient medium, respectively. Then maturation rate, cleavage rate, blastocyst rate were tested to explore the effect of IGF and FBS on bovine oocyte maturation culture and nuclear transfer embryo development. The results showed that adding 40 ng/mL IGF in mature medium and 20% FBS in nutrient medium, maturation rate, cleavage rate, blastocyst rate were significantly increased. The results would lay a foundation for optimizing bovine somatic cell nuclear transfer technology in the future.