Abstract: The main cultivars of Turpan‘Thmopson seedless’and‘Centennial seedless’were taken as testing materials to study the influence of chlorosis on grape growth and development. The indexes of leaf growth increment, chlorophyll content, fruit yield under different degrees of chlorosis were analyzed. The results showed that the chlorosis caused serious harm to grape growth and the yield and quality of fruits. Along with the intensifying of chlorosis, the single leaf weight, leaf length, leaf width and single leaf thickness of these two grape cultivars reduced gradually, and the growth increment of chlorotic grape plants was significantly lower than that of normal plants. The grape with chlorosis had lower yield, and the normal orchard yield of the two cultivars was 2.47 and 2.51 times of chlorotic orchard yield, respectively. The fruit size and soluble solid content of chlorotic grape were significantly lower than that of normal grape. By comparing different extracting methods of chlorophyll content, it was showed that the efficiency of acetone grinding was better than that of ethanol immersion. The occurrence of chlorosis could cause metabolic disorder of photosynthesis of grape leaves and slow plant growth, and severely threaten the healthy development of grape industry in Turpan. Therefore, adopting effective measures to control chlorosis in grape orchard should be the focus of grape production in Turpan.