Effect of PP333,B9 and ABA on Conservation of TARO(Colocasia esculenta L.Schott)Germplasm Resources in vitro

doi:10.11924/j.issn.1000-6850.casb17080002

Abstract

Abstract: The paper aims to extend the preservation time and improve the survival rate of taro tube seedlings.. The taro cluster buds was used as experimental material, and the effect of three kinds of different concentrations of growth inhibitors and different temperature and light conditions on the survival rate of taro tube seedlings were studied.【Method】 The result showed that at normal temperature（25±2）℃ with light condition, the best PP333, ABA and B9 concentrations were 2 mg/L, 2 mg/L and 1.0 mg/L, respectively. At low temperature（5±1）℃with light condition, the best PP333, ABA and B9 concentrations were 1.0 mg/L,1.0 mg/L and 0.5 mg/L. At low temperature（5±1）℃ with dark condition, the best PP333, ABA and B9 concentrations were 1.0 mg/L,1.0 mg/L and 0.5 mg/L. Compared with normal temperature light condition culture, low temperature（5±1）℃ light culture had higher survival rate, and their best concentrations for TARO germplasm in vitro conservation were lower than that of normal temperature light condition culture. Compared with low temperature light culture, dark conditions at low temperature had higher survival rate, and their best concentrations were the same as light conditions at low temperature, but dark conditions at low temperature increased the survival rate. The recovered plantlets grew well and showed no significantly difference in morphology with those of control group. Three kinds of plant growth inhibitors can effectively improve survival rate of TARO tube seedlings.

CLC Number:

S661

References

[1]中科院植物研究所.中国高等植物图鉴[M].第五册.北京:科学出版社,1980.
[2]宋春凤,徐坤.芋的研究进展[J].中国蔬菜,2004,1(3):58-61.
[3]毕可华.芋试管苗离体培养与保存技术研究[J].北方园艺,1998,(2):9-10.
[4]宋明,王晓佳.芋头试管芽的保存研究[J].西南大学学报(自然科学版),1991,13(4):409-412.
[5]徐刚标.植物种质资源离体保存研究进展[J].中南林学院学报,2000,20(4):81-87.
[6]潘瑞炽.植物生理学[M].第五版.北京:高等教育出版社,2004.
[7]植物生长调节剂对马铃薯试管苗生长和保存的影响[J].湖南农业大学学报(自然科学版),2005,31(5):514-517.
[8]张胜珍,客绍英,马作东,等.植物延缓剂PP₃₃₃和B₉对菘蓝试管苗生长的影响[J].西南农业学报,2009,22(5):1428-1431.
[9]洪森荣,尹明华.5种植物生长抑制剂对香果树种质离体保存的影响[J].亚热带植物科学,2009,38(4):18-21.
[10]张石诚.植物化学调控原理与技术[M].北京:中国农业大学出版社,1999:333-437.
[11]郭勇,崔颖,谢秀祯.植物细胞培养技术[M].北京:化学工业出版社,2004:194-203
[12]周逊,向长萍.植物生长调节剂在种质离体保存中的研究进展[J].长江蔬菜,2009,(6):6-7.
[13]赵密珍,刁曼妮,钱亚明,等.多效唑对草莓种质离体保存的影响[J].植物遗传资源学报,2003,4(3):242-244.
[14]王桂兰,陈超,李玉华.延长苹果试管苗保存时间的研究[J].河北林果研究,2002,17(3):239-241.
[15]王秋竹,量文轩.几种植物生长抑制剂在伏山碴种质离体保存中的应用[J]. 吉林农业科技学院学报,2009,18(4):51-53.
[16]董伟清,闭志强,唐军.多效唑对芋种质离体保存影响的初步研究[J].长江蔬菜,2013,24(5):37-39.
[17] Yin M H ,Hong S R.Cryopreservation of Dendrobium candidum Wall. ex Lindl. protocorm-like bodies by encapsulation-vitrification[J]. Plant Cell, Tissue and Organ Culture,2009,98(2):179-185
[18]Cui Y Y,Eun-joo H, Kozai K,et al.et al.Number of air exchanges, sucrose concentration, photosynthetic photo flux,and differences in photoperiod and dark period temperatures affect growth of Rehmannia glutinosa plantlets in vitro[J]. Plant Cell,Tissue and Organ Culture,2000,62:219-226.
[19]蒋泽平,刘根林,倪竞德.多效唑及光温条件对丝石竹试管苗生长的影响[J].江苏林业科技,2000,27(5):29-31.
[20]彭民璋,邓九生,甘霖.油梨种质的离体低温保存[J].果树科学,1996,13(2):96-98.
[21]洪森荣,尹明华,邵兴华.野葛高频植株再生体系相关因子的优化[J].亚热带植物科学, 2008,37(1): 29-32.
[22]赵密珍,刁曼妮,钱亚明,等.多效唑对草莓种质离体保存的影响[J].植物遗传资源学报,2003,4(3):242-244.
[23]张希太.应用多效唑常温保存甘薯试管种质的研究[J].中国生态农业学报,2003,11(1):41-43.

Effect of PP₃₃₃,B₉ and ABA on Conservation of TARO(Colocasia esculenta L.Schott)Germplasm Resources in vitro

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