Abstract: To clarify the nitrogen amount for achieving high quality and high efficiency of different genotypes of spring wheat varieties in black soil, [Method] 2 middle gluten spring wheat varieties‘Nongmai No. 4’‘( NM4’),‘Nongmai No. 5’‘( NM5’), and 2 strong gluten varieties‘Jinqiang No. 7’‘( JQ7’) and‘Jinqiang No. 8’‘( JQ8’) were used as materials in 2016-2017 in the Institute of Crop Sciences, Chinese Academy of Agricultural Sciences. Pot experiments in greenhouse were carried out. The effects of interactions between nitrogen amounts [0 kg/hm2 (N0), 120 kg/hm2 (N120) and 240 kg/hm2 (N240)] and varieties on grain protein and amino acid yield in black soil were studied. [Result] The results showed that there were significant (P <0.05) or very significant (P <0.01) effects of nitrogen amount and variety on grain protein and amino acid yield of spring wheat, and the interaction effect of nitrogen amount×variety was obvious (P <0.05). In terms of nitrogen amount in black soil, N 120 kg/hm2 should be used to obtain the high quality and high efficiency of spring wheat. In terms of variety, we should select strong gluten spring wheat variety‘JQ8’ to obtain more protein and amino acid yield rather than other varieties. In terms of interaction effects of nitrogen amount×variety, the strong gluten spring wheat variety‘JQ7’× nitrogen amount N 240 kg/hm2 and the middle gluten spring wheat variety‘NM5’× nitrogen amount N 240 kg/hm2 could achieve the high quality and high efficiency of wheat in black soil. [Conclusion]The study provides theoretical basis and technical support for the improvement of industry development in spring wheat quality under black soil.