Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2018, Vol. 34 ›› Issue (14): 134-139.doi: 10.11924/j.issn.1000-6850.casb17120018

Special Issue: 生物技术 畜牧兽医

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Fecal Microbial Flora Analysis in MSTN/FGF5 Gene-modified Shaanbei White Cashmere Goat Mediated by CRISPR/Cas9

  

  • Received:2017-12-04 Revised:2018-04-19 Accepted:2018-02-24 Online:2018-05-17 Published:2018-05-17

Abstract: In order to assess the MSTN/FGF5 gene modified Shaanbei cashmere goats on the health of animal themselves and on the safety of environment from the aspect of the changes in microbe population of goat faeces, six different kinds of media were used to count fecal bacteria of the MSTN/FGF5 gene modified and wild-type goats, respectively, microbial flora and significance test analysis was also conducted. Results of microbial flora of feces which were cultured on the different media including Luria-Bertani, Bile Esculin Azide, DHL agar, Mannitol salt selective, Eosin methylene blue agar and TPY plates were as follows: F0, male, 6.35±0.68, 5.89±0.25; 5.32±0.10, 5.27±0.34; 6.64±0.57, 6.71±0.35; 5.03±0.53, 5.32±0.14; 4.12±0.13, 4.15±0.21; 5.34±0.18, 5.25±0.25; female 6.62±0.45, 6.64±0.31; 4.80±0.51, 4.75±0.45; 7.20±0.30, 7.15±0.55; 4.30±0.52, 4.42±0.62; 5.83±0.51, 5.73±0.86; 6.24±0.28, 6.24±0.21。F1: male 6.92±0.12, 7.08±0.04; 4.02±0.64, 3.99±0.51; 6.32±0.15, 6.25±0.30; 5.21±0.28, 5.17±0.20; 3.66±0.50, 3.88±0.67; 4.06±0.50, 3.97±0.46; female 7.12±0.24, 6.96±0.19; 4.15±0.19, 4.25±0.24; 6.45±0.30, 6.56±0.45; 4.97±0.42, 4.87±0.38; 4.04±0.96, 3.96±0.57; 4.10±0.28, 3.85±0.37. Results indicated that in comparison with the control goats, microbe population of various species in feces of MSTN/FGF5 gene modified goats remained unchanged (P>0.05). In summary, this study provided the experimental data and research basis for the safety evaluation of MSTN/FGF5 gene modified Shaanbei cashmere goat.