Abstract: This study aims at the effects of cadmium on seedlings of rapeseed (Brassica napus L.) and the screening of cadmium tolerant lines (varieties). Taking 35 Brassica napus. L lines (varieties) from differentngenetic backgrounds as the materials, we carried out the stress treatments under different concentrations of cadmium (1 mg/L, 5 mg/L and 10 mg/L), respectively; and determined 6 indexes including the relative germination vigor, the seedling rate, the inhibition rate of root length, the inhibition rate of seedling height, the inhibition rate of seedling fresh weight and the inhibition rate of seedling water content, to evaluate the cadmium tolerance in different rapeseed lines (varieties) by using the principal component and subordinate function analysis. The results showed that: with the increase of cadmium concentration, cadmium stress enhanced the germination vigor of most rapeseed lines (varieties), but the seedling rates were inhibited; the inhibition rate of root length and seedling height were raised and the seedling fresh weight and seedling water content decreased. 10 lines (varieties) were selected by comprehensive assessment, among which the material C13095-6 showed the strongest cadmium tolerance. This study provides a reference for screening and breeding