Abstract: For evaluating the efficiency of the method that reselecting from the recombination of maize landraces and their derivatives and investigating new ways to utilize elite landrace resources, we conducted comprehensive evaluations on ''Wo 30'' and 8 recombinant inbred lines based on phenotypic analysis, principal component analysis, correlation analysis and cluster analysis to study the comprehensive evaluation index and method of inbred lines developed from landraces. The results showed that (1) there were abundant genetic variations between ''Wo 30'' and these recombinant inbred lines, the coefficient of variation of each trait was 1.49%-46.79%, and the multiple characters of ''A3'' and ''A4'' were superior to that of ''Wo 30''. (2) the comprehensive evaluation analysis showed that the cumulative contribution rate of 3 principal components was 90.46%, the comprehensive score of the phenotypic traits of the tested materials was varied from -1.414 to 2.550, and the overall scores of ''A4'', ''A3'' and ''A1'' were higher than that of ''Wo 30''. Six phenotypic traits such as the pollinating stage were significantly or highly significantly correlated with the comprehensive score. (3) the genetic distance of the tested materials were varied from 0.78 to 2.045. Three groups were classified when the threshold was selected as 1.414. Overall, the target traits were improved by reselecting from the recombination of ''Worihuang'' and its derivatives, which was beneficial to further explore and utilize the elite genes in the local varieties.