Application of Pig Manure Affects Growth of Vegetable, Residues of Antibiotics and Heavy Metals in Soil

doi:10.11924/j.issn.1000-6850.casb18040140

Abstract

Abstract: To investigate the application effects of pig manure containing multiple antibiotics and heavy metal elements on soil and crops, a field experiment was carried out to study the after- one- year effects of decomposed pig manure and fresh pig manure on tetracycline antibiotics [tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC)] residues, resistant bacteria and heavy metals (Cu, Cd, Ni, Pb, Zn and Cr) in soil. Their effects on soil physical and chemical properties and vegetable yield were also studied. The results showed that the yields of water cabbage, Chinese flowering cabbage, lettuce and green stem vegetable under fresh pig manure (FPM) treatments increased by 11.3%, 3.2%, 75.0% and 6.8% respectively compared with those under decomposed pig manure (CPM) treatments. After one year application of decomposed pig manure (CPM), the number of anti-OTC and anti-TC resistant bacteria in soil was 73.07% and 40.89% higher than that of FPM application, respectively, and the proportion of anti-OTC resistant bacteria to total bacteria was significantly higher than FPM application and CK (163.10% and 307.10%, respectively), the proportion of anti-CTC resistant bacteria to total bacteria was significantly higher than CK (52.3%). The content of TC, OTC, CTC and the total amount of tetracycline antibiotics in soil increased significantly by CPM and FPM. The content of Cd, Cu and Zn in soil of FPM treatments and CPM treatments increased by 52.7%, 33.7% and 29.8% (CPM), 8.7%, 24.1% and 16.9% (FPM), respectively. The content of Cd, Cu and Zn in soil was in order of CPM> FPM>CK. Long-term application of pig manure causes the risk of accumulation of tetracycline antibiotics and heavy metals in soil.

References

[1] Meng F Q, Qiao Y H, Wu W L, et al. Environmental impacts and production performances of organic agriculture in China: A monetary valuation[J]. Journal of Environmental Management, 2017,(188):49-57.
[2] 江青东,韩峰,周小果. 畜禽废弃物资源化利用的现状与发展前景[J]. 现代牧业,2017,1(02):33-36.
[3] 王森,焦瑞峰,马艳华,等. 我国畜禽粪便综合利用途径研究[J]. 河南科技学院学报(自然科学版),2017,(01):20-24.
[4] Van Boeckel T.P., Brower C., Gilbert M., Grenfell B.T., Levin S.A., Robinson T.P., Teillant A., Laxminarayan R. (2015) Global trends in antimicrobial use in food animals. Proc Natl Acad Sci U S A 112:5649-54. DOI: 10.1073/pnas.1503141112.
[5] 楼晨露. 长期定量施用猪粪稻田土壤中典型抗生素及其抗性基因污染研究[D]. 浙江大学, 2016.
[6] 史艳财. 猪粪、沼液农用重金属和抗生素的生态风险及生物调控研究[D]. 华南农业大学, 2016.
[7] Cheng D, Xie Y, Yu Y, et al. Occurrence and Partitioning of Antibiotics in the Water Column and Bottom Sediments from the Intertidal Zone in the Bohai Bay, China[J]. Wetlands, 2016, 36(1):167-179.
[8] Qian M., Wu H., Wang J., Zhang H., Zhang Z., Zhang Y., Lin H., Ma J. (2016) Occurrence of trace elements and antibiotics in manure-based fertilizers from the Zhejiang Province of China. Sci Total Environ 559:174-81. DOI: 10.1016/j.scitotenv.2016.03.123
[9] 沈建忠. 如何正确理解抗生素在动物养殖业上的地位和作用[J]. 兽医导刊, 2017(15):6-8.
[10] 霍艳军. 饲料无抗化——挡不住的大趋势[J]. 饲料与畜牧,2016,(02):9-10.
[11] Tang X., Lou C., Wang S., Lu Y., Liu M., Hashmi M.Z., Liang X., Li Z., Liao Y., Qin W., Fan F., Xu J., Brookes P.C. (2015) Effects of long-term manure applications on the occurrence of antibiotics and antibiotic resistance genes (ARGs) in paddy soils: Evidence from four field experiments in south of China. Soil Biology and Biochemistry 90:179-187. DOI: 10.1016/j.soilbio.2015.07.027.
[12] Zhang H., Zhou Y., Huang Y., Wu L., Liu X., Luo Y. (2016) Residues and risks of veterinary antibiotics in protected vegetable soils following application of different manures. Chemosphere 152:229-237. DOI: 10.1016/j.chemosphere.2016.02.111.
[13] 周永辉, 张翔, 赵子方,等. 我国畜禽肉中抗生素残留的原因及对策[J]. 食品安全导刊, 2015(9X):148-149.
[14] 史艳财. 猪粪、沼液农用重金属和抗生素的生态风险及生物调控研究[D].华南农业大学,2016.
[15] 郭玉晖. 施加畜禽粪肥对蔬菜中抗生素抗性内生细菌的影响[D].河南师范大学,2015.
[16] Diop M, Howsam M, Diop C, et al. Spatial and seasonal variations of trace elements concentrations in liver and muscle of round Sardinelle (Sardinella aurita) and Senegalese sole (Solea senegalensis) along the Senegalese coast[J]. Chemosphere, 2015, 144:758-766.
[17] 司晓薇. 环境中磺胺类药物的残留、形态转化及生态毒性研究[D]. 河南师范大学, 2016.
[18] 黄新灿, 章明奎. 长期施用猪粪源有机肥对蔬菜-土壤系统重金属积累的影响[J]. 农学学报, 2016, 6(4):30-35.
[19] 王瑾, 韩剑众. 饲料中重金属和抗生素对土壤和蔬菜的影响[J]. 生态与农村环境学报, 2008, 24(4):90-93.
[20] 李向东,陈烨. 喀斯特地区高硬度水质和重金属污染对人体健康的影响[J]. 微量元素与健康研究,2014(2):71-73.
[21] 茹淑华, 张国印, 杨军芳,等. 鸡粪和猪粪对小麦生长及土壤重金属累积的影响[J]. 华北农学报, 2015, 30(s1):494-499.
[22] 范学臻, 胡文友, 冒维维,等. 不同有机肥施用对小青菜产量和土壤重金属含量的影响[J]. 现代农业科技, 2015(20):174-174.
[23] 余天红, 黎华寿, 贺鸿志,等. 施用不同预处理猪粪对菜园土壤pH、胡敏酸含量及Cu、Zn活性的影响[J]. 农业环境科学学报, 2016, 35(4):785-791.
[24] 董占荣,陈一定,林咸永,等.杭州市郊规模化养殖场猪粪的重金属含量及其形态[J].浙江农业学报,2008,20(1):35-39.
[25] 中华人民共和国国家标准农用污泥中污染物控制标准[J]. 农业环境科学学报,1988,(03):47-48.
[26] 欧阳达. 长期施肥对农田土壤肥力及重金属化学行为的影响[D]. 湖南师范大学, 2015.
[27] 张昌爱,单洪涛,辛淑荣,等. 猪粪发酵与否对土壤性质的影响差异[J]. 农业科技与信息,2016,(26):95+97.
[28] 鲍陈燕. 猪粪对抗生素在农田系统中行为的影响[D]. 浙江大学, 2016.
[29] 崔亚男, 张旭辉, 刘晓雨,等. 不同猪粪施用方式对小白菜生长、产量及品质的影响[J]. 南京农业大学学报, 2017, 40(2):281-286.
[30] 王瑞, 魏源送, 赵高峰,等. 四环素类抗生素和铜复合污染对猪粪厌氧消化的影响[J]. 生态毒理学报, 2015, 10(5):108-114.
[31] 郑宁国, 黄南, 王卫卫,等. 高温堆肥过程对猪粪来源抗生素抗性基因的影响[J]. 环境科学, 2016, 37(5):1986-1992.ZHENG Ning-guo, HUANG Nan, Wang Wei-wei, et al. Effects of thermophilic composting on Antibiotic Resistance Genes (ARGs) of swine manure source[J]. Environmental Science, 2016, 37 (5): 1986-1992.
[32] 雷慧宁. 规模化猪场废水处理工艺中抗生素和重金属残留及其生态风险[D]. 华东师范大学, 2016.
[33] 郑宁国. 猪粪堆肥对抗生素抗性基因的影响及微生物群落变化的初步探究[D]. 西北大学, 2016.
[34] 勾长龙, 王雨琼, 张喜庆,等. 高温堆肥对猪粪中四环素类抗生素及抗性基因的影响[J]. 环境科学学报, 2017, 37(4):1454-1460.
[35] 肖礼, 黄懿梅, 赵俊峰,等. 外源菌剂对猪粪堆肥质量及四环素类抗生素降解的影响[J]. 农业环境科学学报, 2016, 35(1):172-178.
[36] 李晓阳. 兽用抗生素强力霉素与土壤微生物的互作效应[D]. 华南农业大学, 2016.
[37] 曹译戈, 杨娟, 王昌全,等. 猪粪施用对稻麦轮作体系土壤碳组分含量及动态变化的影响[J]. 土壤通报, 2016, 47(4):935-943.
[38] 刘源. 增施猪粪与秸秆对水稻养分吸收及土壤酸化的影响[D]. 海南大学, 2016.
[39] 孙刚. 畜禽粪便中四环素类抗生素检测分析及其在堆肥中的降解研究[D].合肥工业大学,2010.
[40] 李平, 郎漫, 李煜姗,等. 不同施肥处理对黑土硝化作用和矿化作用的影响[J]. 农业环境科学学报, 2015, 34(7):1326-1332.
[41] 史艳财. 猪粪、沼液农用重金属和抗生素的生态风险及生物调控研究[D].华南农业大学,2016.
[42] 刘健龙. 养猪粪污中重金属Cu的去除技术研究[D]. 重庆大学, 2016.
[43] Wang J., Lin H., Sun W., Xia Y., Ma J., Fu J., Zhang Z., Wu H., Qian M. Variations in the fate and biological effects of sulfamethoxazole, norfloxacin and doxycycline in different vegetable-soil systems following manure application. J Hazard Mater 304:49-57. DOI: 10.1016/j.jhazmat.2015.10.038.
[44] Xu, Y., W. Yu, Q. Ma and H. Zhou Interactive effects of sulfadiazine and Cu(II) on their sorption and desorption on two soils with different characteristics. Chemosphere 138: 701-707. doi: 10.1016/j.chemosphere.2015.07.049
[45] 贾武霞. 畜禽粪便施用对土壤中重金属累积及植物有效性影响研究[D]. 中国农业科学院,2016.
[46] 吴耀,季淑枫,章林英,等. 猪粪与茶树修剪物对土壤Pb和Cd有效性的影响[J]. 中国园艺文摘,2014,30(08):31-33.