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Chinese Agricultural Science Bulletin ›› 2020, Vol. 36 ›› Issue (28): 78-83.doi: 10.11924/j.issn.1000-6850.casb20191000710

Special Issue: 生物技术

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Effects of Nitrogen Fertilizer Application Rates on Nitrogen Metabolism Physiology of Euonymus kiautschovicus

Xu Jinxian()   

  1. Shanxi Guandishan State-owned Forestry Administration, Lvliang Shanxi 032104
  • Received:2019-10-11 Revised:2020-01-08 Online:2020-10-05 Published:2020-09-23

Abstract:

The paper aims to study the effect of nitrogen on Euonymus kiautschovicus nitrogen metabolism physiology, clarify the change mechanism of related enzymes activity after nitrogen fertilizer application, and provide a theoretical basis for scientific nitrogen fertilizer application in seedling raising. The test was conducted under field condition, 4 treatments were set with the application amount of nitrogen fertilizer of 0 g/plant(J1, CK), 9 g/plant(J2), 18 g/plant(J3), and 27 g/plant(J4), repeating 3 times. The results showed that: the GOT activity of J3 was higher than that of J1 by 64.84%, 70.77%, 58.52%, 47.33%, 57.20% respectively from May to September, there was no significant difference between J4 and J3; the GPT activity of J3 was higher than that of J2 by 18.00%, 30.79%, 16.47%, 16.83% respectively from June to September with significant difference; GS activity of J3 was significantly higher than that of J2 from July and September, but there was no significant difference between J2, J3 and J4 in May and June, and GS activity of J2 was significantly higher than that of the control; NR activity of J3 was higher than that of J2 by 20.47%, 31.66%, 27.32%, 23.50%, 34.90% respectively from May to September with significant difference, while there was no significant difference between J3 and J4. The content of nitrate nitrogen increased by 73.76%, 53.90%, 85.95% respectively from May to July compared with that of control and the difference was significant, while there was no significant difference between J2, J3 and J4 from August to September; the soluble protein content of J3 was higher than that of J2 by 15.90%, 20.09%, 9.04% respectively from June to August and the difference was significant. According to the comprehensive analysis, J3 treatment could significantly increase the activity of nitrogen metabolism-related enzymes, nitrate nitrogen content and soluble protein content in leaves of Euonymus kiautschovicus, and the effect was better than J2 and J4 treatment.

Key words: nitrogen fertilizer, nitrogen metabolic enzymes, Euonymus kiautschovicus, soluble protein, nitrate nitrogen

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