An InDel-spot Molecular Marker for the Spotted Trait in Melon: Development and Application

doi:10.11924/j.issn.1000-6850.casb20191200970

Abstract

Abstract:

The purpose is to improve the selecting efficiency of breeding materials with the spotted and the non-spotted trait in melons. The authors conducted the research with the spotted and non-spotted melons provided in field, and developed the linked InDel-spot molecular marker near the gene controlling the melon spotted trait in chromosome 2. Twenty melons were genotyped with the InDel-spot maker. The results showed that the size of spotted band was about 211 bp, while the non-spotted band was about 219 bp, with 95% of compliance rate. The InDel-spot marker was further used to identify the spotted and non-spotted trait of 2 (F₂) and 5 (F₃) populations. Among them, the detection accuracy of Qb978 (F₂), Qb931 (F₃) and Qb932 (F₃) was 96.6%, 91.7% and 83.3%, respectively. The results indicate that InDel-spot marker has a great application value in the identification of the spotted and non-spotted trait, which could raise the selecting efficiency of breeding materials and shorten the breeding cycle.

Key words: melon, spotted trait, non-spotted trait, InDel-spot molecular marker, breeding cycle

CLC Number:

S333.6

Xie Meiting, Liu Li, Hua Deping. An InDel-spot Molecular Marker for the Spotted Trait in Melon: Development and Application[J]. Chinese Agricultural Science Bulletin, 2020, 36(35): 37-41.

Figures/Tables 7

References 27

[1]	林德佩. 中国栽培甜瓜植物的起源、分类及进化[J]. 中国瓜菜, 2010,23(4):34-36.
[2]	吕建春. 薄皮甜瓜(Cucumis melo L. var. chinensis Pangalo)果皮花斑的遗传分析及基因定位[D]. 北京:中国农业科学院, 2018.
[3]	刘晓娟, 杨玉珍, 周红杰, 等. 园林植物彩斑现象形成机理及原因[J]. 北方园艺, 2014(3):182-186.
[4]	尚啸, 王健, 李琴, 等. 植物花斑形成分子机理研究进展[J]. 园艺学报, 2014,41(7):1485-1494.
[5]	Campitelli B. Leaf variegation is associated with reduced herbivore damage in Hydrophyllum virginianum[J]. Botany-botanique, 2008,86(3):306-313. doi: 10.1139/B07-139 URL
[6]	Esteban R, Fernandez-Marin B, Becerril J M, et al. Photoprotective implications of leaf variegation in E. dens-canis L. and P. officinalis L[J]. plant physiology, 2008,165(12):1255-1263. doi: 10.1104/pp.114.239699 URL
[7]	Scopece G. Pollinator attraction in Anacamptis papilionacea (Orchidaceae): a food or a sex promise[J]. Plant Species Biology, 2009,24(2):109-114. doi: 10.1111/psb.2009.24.issue-2 URL
[8]	胡建斌, 马双武, 李建吾, 等. 国外甜瓜种质资源形态性状遗传多样性分析[J]. 植物学报, 2013,48(1):42-51. doi: 10.3724/SP.J.1259.2013.00042 URL
[9]	林德佩. 中国栽培甜瓜植物的起源、分类及进化[J]. 中国瓜菜, 2010,23(4):34-36.
[10]	Pitrat M. Some comments on infraspecific classification of melon[M]. Acta Horticultural, 2000: 510.
[11]	林德佩. 新疆野生甜瓜的研究[J]. 八一农学院学报, 1984(1):50-52.
[12]	新疆甜瓜西瓜资源调查组. 新疆甜瓜西瓜志[M]. 乌鲁木齐: 新疆人民出版社, 1985.
[13]	华德平, 刘莉, 杨旭辉, 等. 一种用于鉴定甜瓜果肉颜色的InDel标记引物及分子标记方法[P]. 2019-03-26.
[14]	Tzuri G. A’golden’ SNP in CmOr governs the fruit flesh color of melom(Cucumis melo)[J]. plant Journal, 2015,82(2):267-279.
[15]	张乔玲, 华德平, 杨旭辉, 等. 甜瓜单性雌花共显性分子标记的开发及应用[J]. 分子植物育种, 2019,17(9):2932-2938.
[16]	李凤梅, 张洪亮, 崔健, 等. 甜瓜单性花相关基因CmACS-7的分子标记开发与应用[J]. 分子植物育种, 2018,16(14):4715-4720.
[17]	Harel-Beja R, Tzuri G, Portnoy V, et al. A genetic map of melon highly enriched with fruit quality QTLs and EST markers, including sugar and carotenoid metabolism genes[J]. Theoretical and Applied Genetics, 2010,121(3):511-533. doi: 10.1007/s00122-010-1327-4 URL pmid: 20401460
[18]	黄琼. 新疆甜瓜果实绿色条纹性状AFLP标记的初步筛选[D]. 乌鲁木齐:新疆大学, 2008.
[19]	Danin-Poleg Y. Tadmor Y. Construction of a genetic map of melon with molecular markers and horticultural traits, and localization of genes associated with ZYMV resistance[J]. Euphytica, 2002,25(3):373-384.
[20]	Périn C. Genetic control and linkages of some fruit characters in melon[J]. Cucurbit Genetic Cooperative Report. 1999,22(3):16-18.
[21]	Murray P. Gene List for melon[J]. Cucurbit Genetics Cooperative Report, 2006,38(28-29):142-163.
[22]	Périn C. A reference map of Cucumis melo based on two recombinant inbred line populations[J]. Theoretical and Applied Genetics, 2002,104(6-7):1017-1034. doi: 10.1007/s00122-002-0864-x URL pmid: 12582608
[23]	Murray G, Thompson F. Rapid isolation of high molecular weight plant DNA[J]. nucleic acids research, 1980,8(19):4321-4325. doi: 10.1093/nar/8.19.4321 URL pmid: 7433111
[24]	杨光华, 范荣, 杨小锋, 等. 甜瓜果实颜色3个质量性状基因的定位[J]. 园艺学报, 2014,41(5):898-906.
[25]	Paris. Gene for broad, contiguous dark stripes in cocozelle squash (Cucurbita pepo)[J]. Euphytica, 2000,115(3):191-196.
[26]	Paris. Genetic control of irregular striping, a new phenotype in Cucurbita pepo.[J]. Euphytica, 2003,129(1):119-126.
[27]	Lou L. Qualitative inheritance of external fruit traits in watermelon[J]. Hotscience, 2016,51(5).

编号	田间编号	果皮类型	变种类型
1	Qa159	花皮	var.chinensis
2	Qa346	花皮	var.chinensis
3	Qa155	花皮	var.chinensis
4	Qa168	花皮	var.chinensis
5	Qa184	花皮	var.chinensis
6	Qa204	花皮	var.chinensis
7	Qa213	花皮	var.chinensis
8	Qa300	花皮	var.chinensis
9	Qa307	花皮	var.chinensis
10	Qa58	黄绿皮	var.chinensis
11	Qa347	黄绿皮	var.chinensis
12	Qa1	白皮	var.chinensis
13	Qa52	黄皮	var.chinensis
14	Qa86	深绿皮	var.chinensis
15	Qa96	绿皮	var.chinensis
16	Qa 420	灰绿皮	var.chinensis
17	Qa384	墨绿皮	var.chinensis
18	Qa392	黄绿皮	var.chinensis
19	Qa 332	灰黄绿皮	var.chinensis
20	Qa12	白皮	var.chinensis

编号	田间编号	果皮类型	变种类型
1	Qa159	花皮	var.chinensis
2	Qa346	花皮	var.chinensis
3	Qa155	花皮	var.chinensis
4	Qa168	花皮	var.chinensis
5	Qa184	花皮	var.chinensis
6	Qa204	花皮	var.chinensis
7	Qa213	花皮	var.chinensis
8	Qa300	花皮	var.chinensis
9	Qa307	花皮	var.chinensis
10	Qa58	黄绿皮	var.chinensis
11	Qa347	黄绿皮	var.chinensis
12	Qa1	白皮	var.chinensis
13	Qa52	黄皮	var.chinensis
14	Qa86	深绿皮	var.chinensis
15	Qa96	绿皮	var.chinensis
16	Qa 420	灰绿皮	var.chinensis
17	Qa384	墨绿皮	var.chinensis
18	Qa392	黄绿皮	var.chinensis
19	Qa 332	灰黄绿皮	var.chinensis
20	Qa12	白皮	var.chinensis

F₂群体	F₂代群体株树
F₂群体	211 bp植株	219 bp植株	无条带植株	总计
Qb977	112	66	14	192
Qb978	67	20	9	96

F₂群体	F₂代群体株树
F₂群体	211 bp植株	219 bp植株	无条带植株	总计
Qb977	112	66	14	192
Qb978	67	20	9	96