Pyruvate Decarboxylase (Pdc6) Gene Cloning and Bioinformatics Analysis in Saccharomyces cerevisiae

doi:10.11924/j.issn.1000-6850.casb2020-0296

Abstract

Abstract:

The aims are to study the structure and function of pyruvate decarboxylase (Pdc6) in Saccharomyces cerevisiae, and clone pdc6 gene in S. cerevisiae H5. A pair of 20 bp primers was designed by Primer5.0 software. The pdc6 gene was cloned using the genomic DNA of S. cerevisiae H5 as template, and the cloned sequence was subsequently verified and analyzed by bioinformatics method. The results show that the sequence length is 1692 bp with a completely open reading frame (ORF) without deletion. Besides, the Pdc6 protein contains 563 amino acid residues and the number of acidic amino acid is almost the same as that of the basic amino acid residues. The theoretical isoelectric point of Pdc6 is 5.8 and the maximum hydrophobicity is 2.32. Subcellular localization predicts that it is located in extracellular matrix and belongs to acidic protein, and the spatial structure model is predicted. This study shows that the structure of Pdc6 protein is similar to the Pdc1 protein and the Pdc5 protein. The bioinformatically analysis of the gene sequence of pyruvate decarboxylase (Pdc6) encoded by S. cerevisiae H5 can provide a theoretical basis for the subsequent study of gene sequence selection for single gene knockout.

Key words: Saccharomyces cerevisiae, bioinformatics analysis, clone, pyruvate decarboxylase (Pdc6) gene, ORF

CLC Number:

S216.2

Wang Changli, Liao Wei, Ye Guangbin, Ge Jingping, Liu Lei, Ma Yujian, Huang Xia, Bin Xiaoyun. Pyruvate Decarboxylase (Pdc6) Gene Cloning and Bioinformatics Analysis in Saccharomyces cerevisiae[J]. Chinese Agricultural Science Bulletin, 2021, 37(9): 103-108.

Figures/Tables 2

References 27

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