Abstract:

By studying the fermentation characteristics of strain, we aim at obtaining a good 2,3-butanediol (2,3-BD) producing strain. Four strains of Saccharomyces cerevisiae W5/W5-$\Delta$pdc1/W5-$\Delta$pdc5/W141 were used as materials in this study, the yield of acetoin and BDH enzyme activity were detected with HPLC, in order to select the most optimum starting strain for producing 2,3-BD, and the starting strain producing 2,3-BD was added with different concentrations of acetoin, and the changes of acetoin, glucose and 2,3-BD production with fermentation time were determined. The result showed that S. cerevisiae W5/W141 were selected as the optimum strain, the optimum acetoin concentration of S. cerevisiae W5 was 12 g/L, and the concentration of 2,3-BD reached the maximum at 72 h, and it was 2.49 ± 0.02 g/L. The optimum acetoin concentration of S. cerevisiae W141 was 8 g/L, and the concentration of 2,3-BD reached the maximum at 72 h, and it was 1.56 ± 0.04 g/L, the strain grew well after adding acetoin, and the acetoin produced by S. cerevisiae W5/W141 was completely consumed before the end of fermentation, and the glucose produced by S. cerevisiae W5/W141 was almost exhausted at 24 h. This provides an idea for the research and utilization of renewable resources such as 2,3-BD, and also lays a theoretical foundation for S. cerevisiae produces 2,3-BD.

Key words: 2,3-butanediol, acetoin, Saccharomyces cerevisiae, strain growth, HPLC