Effects of Antagonistic Bacillus on the Main Active Components of Astragalus membranaceus var. mongholicus

doi:10.11924/j.issn.1000-6850.casb2020-0399

Abstract

Abstract:

The effects of antagonistic Bacillus SXKF16-1 (Bacillus atrophaeu) and SXKF16-2 (B. methylophicus) on the main active components of Astragalus membranaceus var. mongholicus were investigated so as to provide a basis for the development of multi-functional preparations. Healthy A. membranaceus var. mongholicus seedlings were root-irrigated with the fermentation of antagonistic Bacillus, and ten effective components were determined by HPLC-UV-ELSD. There was a good linear relationship (r≥0.9950) in the corresponding linear range of the respective standard curve of calycosin-7-O-β-D-glucoside, ononin, (6aR,11aR)-9,10-dimethoxyptercarpan, 8,2'-dihydroxy-3,4-dimethoxyisoflavan-7-O-β-D-glucopyranoside, calycosin, formononetin, astragaloside Ⅳ, Ⅲ, Ⅱ, Ⅰ, and thus the test results were reliable. Treated with antagonistic Bacillus SXKF16-1 and SXKF16-2, the contents of such A. membranaceus var. mongholicus active components as calycosin-7-O-β-D-glucoside, ononin, (6aR,11aR)-9,10-dimethoxyptercarpan, 8,2'-dihydroxy-3,4-dimethoxyisoflavan-7-O-β-D-glucopyranoside, calycosin, formononetin, astragaloside Ⅱ and Ⅳ showed no significant change, but the contents of astragaloside Ⅰ and Ⅲ increased significantly. Compared with the control group, the content of total flavonoids in the two treatment groups had no significant change, while the content of total saponins increased significantly. Antagonistic Bacillus SXKF16-1 and SXKF16-2 have no negative effect on the content of main active components of A. membranaceus var. mongholicus, and can promote the accumulation of some saponins; and therefore have the potential of being developed into multi-functional preparations.

Key words: Antagonistic Bacillus, Astragalus membranaceus var. mongholicus, root rot, HPLC-UV-ELSD, flavonoids, saponins

CLC Number:

S182

Guo Jie, Tian Haijiao, Qin Xuemei, Zhao Xiaoxia, Gao Fen. Effects of Antagonistic Bacillus on the Main Active Components of Astragalus membranaceus var. mongholicus[J]. Chinese Agricultural Science Bulletin, 2021, 37(19): 84-89.

Figures/Tables 4

References 29

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成分	系列质量浓度/(mg/mL)	回归方程	线性范围/μg	r²
毛蕊异黄酮葡萄糖苷	0.935、0.312、0.078、0.039、0.019、0.010	Y=55753.85X+547.91	0.298~18.700	1.0000
芒柄花苷	0.680、0.340、0.170、0.085、0.042、0.021	Y=53114X+63560	0.212~6.800	0.9999
紫檀烷苷	0.681、0.340、0.170、0.085、0.043、0.021	Y=46359X+60442	0.213~6.810	0.9999
异黄烷苷	0.300、0.150、0.038、0.019、0.010	Y=36788X+13584	0.094~6.010	0.9991
毛蕊异黄酮	0.276、0.138、0.069、0.034、0.017、0.009	Y=98675X-42582	0.082~5.220	0.9991
芒柄花素	0.627、0.314、0.157、0.078、0.039、0.020	Y=98481X-26826	0.196~6.270	0.9993
黄芪皂苷Ⅳ	0.245、0.061、0.015、0.008、0.004	Y=1.674X+1.788	0.076~4.895	0.9950
黄芪皂苷Ⅲ	0.250、0.125、0.031、0.008、0.004	Y=1.268X+2.584	0.078~2.500	0.9993
黄芪皂苷Ⅱ	0.291、0.146、0.073、0.036、0.018	Y=1.387X+2.414	0.091~5.820	0.9950
黄芪皂苷Ⅰ	0.986、0.493、0.246、0.123、0.062、0.031	Y=1.055X+3.382	0.616~19.720	0.9990

成分	系列质量浓度/(mg/mL)	回归方程	线性范围/μg	r²
毛蕊异黄酮葡萄糖苷	0.935、0.312、0.078、0.039、0.019、0.010	Y=55753.85X+547.91	0.298~18.700	1.0000
芒柄花苷	0.680、0.340、0.170、0.085、0.042、0.021	Y=53114X+63560	0.212~6.800	0.9999
紫檀烷苷	0.681、0.340、0.170、0.085、0.043、0.021	Y=46359X+60442	0.213~6.810	0.9999
异黄烷苷	0.300、0.150、0.038、0.019、0.010	Y=36788X+13584	0.094~6.010	0.9991
毛蕊异黄酮	0.276、0.138、0.069、0.034、0.017、0.009	Y=98675X-42582	0.082~5.220	0.9991
芒柄花素	0.627、0.314、0.157、0.078、0.039、0.020	Y=98481X-26826	0.196~6.270	0.9993
黄芪皂苷Ⅳ	0.245、0.061、0.015、0.008、0.004	Y=1.674X+1.788	0.076~4.895	0.9950
黄芪皂苷Ⅲ	0.250、0.125、0.031、0.008、0.004	Y=1.268X+2.584	0.078~2.500	0.9993
黄芪皂苷Ⅱ	0.291、0.146、0.073、0.036、0.018	Y=1.387X+2.414	0.091~5.820	0.9950
黄芪皂苷Ⅰ	0.986、0.493、0.246、0.123、0.062、0.031	Y=1.055X+3.382	0.616~19.720	0.9990

药效成分	CK	SXKF16-1	SXKF16-2
毛蕊异黄酮葡萄糖苷	0.535±0.069a	0.576±0.117a	0.531±0.035a
芒柄花苷	0.154±0.018a	0.165±0.027a	0.158±0.012a
紫檀烷苷	0.228±0.016a	0.252±0.037a	0.274±0.026a
异黄烷苷	0.154±0.027a	0.186±0.030a	0.204±0.030a
毛蕊异黄酮	0.272±0.056ab	0.303±0.016b	0.224±0.024a
芒柄花素	0.108±0.024ab	0.122±0.007b	0.085±0.013a
黄芪皂苷Ⅰ	14.27±0.000a	31.218±1.255b	30.01±1.167b
黄芪皂苷Ⅱ	1.409±0.310a	1.797±0.174a	1.553±0.096a
黄芪皂苷Ⅲ	2.234±0.295a	3.059±0.416b	2.984±0.372b
黄芪皂苷Ⅳ	1.13±0.233a	1.096±0.114a	0.999±0.215a

药效成分	CK	SXKF16-1	SXKF16-2
毛蕊异黄酮葡萄糖苷	0.535±0.069a	0.576±0.117a	0.531±0.035a
芒柄花苷	0.154±0.018a	0.165±0.027a	0.158±0.012a
紫檀烷苷	0.228±0.016a	0.252±0.037a	0.274±0.026a
异黄烷苷	0.154±0.027a	0.186±0.030a	0.204±0.030a
毛蕊异黄酮	0.272±0.056ab	0.303±0.016b	0.224±0.024a
芒柄花素	0.108±0.024ab	0.122±0.007b	0.085±0.013a
黄芪皂苷Ⅰ	14.27±0.000a	31.218±1.255b	30.01±1.167b
黄芪皂苷Ⅱ	1.409±0.310a	1.797±0.174a	1.553±0.096a
黄芪皂苷Ⅲ	2.234±0.295a	3.059±0.416b	2.984±0.372b
黄芪皂苷Ⅳ	1.13±0.233a	1.096±0.114a	0.999±0.215a