Effects of Low Temperature on Saponin Content and Photosynthetic Physiology of Panax notoginseng in Different Ages

doi:10.11924/j.issn.1000-6850.casb20191200999

Abstract

Abstract:

To clarify the influence of low temperature on the total saponins and the photosynthetic physiology of Panax notoginseng with different growing years, HPLC method was used to analyze the content of total saponins in root of P. notoginseng treated with 10, 15 and 20℃ for 30 days, and measured the photosynthetic characteristics, chlorophyll content and malondialdehyde (MDA) of P. notoginseng tablets of two ages at three temperatures. The results showed that the content of total saponins in three years P. notoginseng treated with 20℃ was the highest, while that with 15℃ was the lowest. The content of chlorophyll a (Chla) and chlorophyll b (Chlb) was the highest at 10℃. The difference between Chlb at 15℃ and 20, 10℃ was significant. The net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr) of leaves decreased with the decrease of temperature. The difference between Pn at 20℃ and 15℃ was significant, and that at 10℃ was very significant. Gs and Tr at 20℃ were significantly different from 15, 10℃. MDA increased first and then decreased with the decrease of temperature. Intercellular CO₂ concentration/ ambient CO₂ concentration (Ci/Ca) increased with the decrease of temperature, and the difference between 10℃ and 15, 20℃was significant. The content of total saponins of two years P. notoginseng treated with 20℃ was the highest and the content of total saponins treated with 15℃ was the lowest. Both Chla and Chlb increased first and then decreased with the decrease of temperature. Both Pn and Gs decreased with the decrease of temperature, and Tr decreased first and then increased with the decrease of temperature. MDA increased with the decrease of temperature. Ci/Ca increased with temperature decrease. Therefore, the effect of low temperature on the accumulation of P. notoginseng root saponins is not significant, and 20℃ is the most optimum temperature for the accumulation of P. notoginseng root saponins, but low temperature can inhibit P. notoginseng photosynthesis.

Key words: Panax notoginseng, saponins, correlation, low temperature, photosynthesis

CLC Number:

R282.2

Zhou Runze, Wang Yihao, Ma Xiaohui, Cui Zhiying, Zheng Guowei, Xu Furong. Effects of Low Temperature on Saponin Content and Photosynthetic Physiology of Panax notoginseng in Different Ages[J]. Chinese Agricultural Science Bulletin, 2020, 36(34): 71-75.

Figures/Tables 2

References 36

[1]	李时珍. 本草纲目(校点本第二册)[M]. 北京: 人民卫生出版社, 1982: 767.
[2]	孟祥霄, 黄林芳, 董林林, 等. 三七全球产地生态适宜性及品质生态学研究[J]. 药学学报, 2016,51(9):1483-1493.
[3]	官会林, 陈昱君, 朱海春, 等. 三七病株根际土壤微生物特征研究[J]. 农业与技术, 2006,25(6):56-58.
[4]	周维佳, 普绍珍, 董玲, 等. 基于主成分分析法的文山三七产业发展状况研究[J]. 云南科技管理, 2015,28(1):72-76.
[5]	殷昌青, 邓海山, 程健. 三七对血液、心脑血管和中枢神经系统的药理作用研究概述[J]. 科技信息, 2010,29(8):50-51.
[6]	夏鹏国, 张顺仓, 梁宗锁, 等. 三七化学成分的研究历程和概况[J]. 中草药, 2014,45(17):2564-2570.
[7]	张毅, 戴胜云, 徐冰, 等. 三七总皂苷抗心肌缺血再灌注损伤的药理研究进展[J]. 江苏中医药, 2017,49(1):82-85.
[8]	唐国政. 三七产业发展的情报分析研究[D]. 昆明:云南大学, 2018: 23-24.
[9]	郭旭初. 健康中国视角下的三七产业发展战略设计[J]. 中共云南省委党校学报, 2018,19(6):126-130.
[10]	孙雪婷, 李磊, 龙光强, 等. 三七连作障碍研究进展[J]. 生态学杂志, 2015,34(3):885-893.
[11]	张子龙, 李凯明, 杨建忠, 等. 轮作对三七连作障碍的消减效应研究[J]. 西南大学学报:自然科学版, 2015,37(8):39-46.
[12]	刘立红, 刘英, 王芬, 等. 云南三七产业发展现状及发展建议[J]. 中国现代中药, 2017,19(9):1331-1335.
[13]	Mackey Katherine R M, Paytan Adina, Caldeira Ken, et al. Effect of Temperature on Photosynjournal and Growth in Marine Synechococcus spp.[J]. Plant physiology, 2013,163(2). doi: 10.1104/pp.113.223560 URL pmid: 23969600
[14]	徐克章, 武志海, 张美善, 等. 人参、西洋参叶片光合作用的温度特性[J].吉林农业大学学报,2002(3):7-10.
[15]	Hu W H, Wu Y, Zeng J Z, et al. Chill-induced inhibition of photosynjournal was alleviated by 24-epibrassinolide pretreatment in cucumber during chilling and subsequent recovery[J]. Photosynthetica, 2010,48(4):537-544. doi: 10.1007/s11099-010-0071-y URL
[16]	Theocharis Andreas, Clément Christophe, Barka Essaïd Ait. Physiological and molecular changes in plants grown at low temperatures[J]. Planta, 2012,235(6):1091-1105. doi: 10.1007/s00425-012-1641-y URL pmid: 22526498
[17]	赵平, 孙谷畴, 曾小平, 等. 两种生态型榕树的叶绿素含量、荧光特性和叶片气体交换日变化的比较研究[J].应用生态学报,2000(3):327-332.
[18]	国家药典委员会. 中华人民共和国药典(第一部)[M]. 北京: 中国医药科技出版社, 2015: 240-241.
[19]	Zheng G, Chen J, Li W. Impacts of CO₂ elevation on the physiology and seed quality of soybean[J]. Plant Diversity, 2020,42(1):44-51. URL pmid: 32140636
[20]	Minocha R, Martinez G, Lyons B, et al. Development of a standardized methodology for quantifying total chlorophyll and carotenoids from foliage of hardwood and conifer tree species[J]. Canadian Journal of Forest Research, 2009,39(4):849-861. doi: 10.1139/X09-015 URL
[21]	Guo H B, Cui X M, An N, et al. Sanchi ginseng (Panax notoginseng (Burkill) FH Chen) in China: distribution, cultivation and variations[J]. Genet Resour Crop Evol, 2010,57(3):453-460. doi: 10.1007/s10722-010-9531-2 URL
[22]	黄璐琦, 郭兰萍. 环境胁迫下次生代谢产物的积累及道地药材的形成[J].中国中药杂志,2007(4):277-280.
[23]	黄璐琦, 陈美兰, 肖培根. 中药材道地性研究的现代生物学基础及模式假说[J]. 中国中药杂志, 2004,29(6):494.
[24]	Frischknecht Peter M, Katharina Schuhmacher, Heinz Müller-Schärer, et al. Phenotypic Plasticity of Senecio vulgaris from Contrasting Habitat Types: Growth and Pyrrolizidine Alkaloid Formation[J]. J Chem Ecol, 2001,27(2). doi: 10.1023/a:1005692502595 URL pmid: 14768810
[25]	Barto E Kathryn, Don Cipollini. Testing the optimal defense theory and the growth-differentiation balance hypojournal in Arabidopsis thaliana[J]. Oecologia, 2005,146(2). doi: 10.1007/s00442-005-0189-y URL pmid: 16025350
[26]	James E Byers. Effects of body size and resource availability on dispersal in a native and a non-native estuarine snail[J]. J Exp Mar Bio Ecol, 2000,248(2).
[27]	武维华. 植物生理学(第二版)[M]. 北京: 科学出版社, 2008: 226-233.
[28]	杨雪梅, 刘旭, 刘艳山, 等. 高效液相色谱法测定三七中三七皂苷R₁及人参皂苷Rg₁、Rb₁的含量[J].时珍国医国药, 2005(8):710-711.
[29]	朱玉洁, 吴雨霏. 气象灾害对文山三七种植的影响及防御对策[J].吉林农业,2019(4):99.
[30]	金花, 王斌, 戴思兰, 等. 北京引种7种常绿阔叶植物的抗寒适应性研究[J].湖南农业科学,2011(1):121-125.
[31]	左应梅, 张金渝, 杨天梅, 等. 温度胁迫对三七光合特性及生理指标的影响[J]. 南方农业学报, 2017,48(12):2145-2151.
[32]	陈奇, 袁金海, 孙万仓, 等. 低温胁迫下白菜型冬油菜与春油菜叶片光合特性及内源激素变化比较[J]. 中国油料作物学报, 2017,39(1):37-46.
[33]	Hu W H, Wu Y, Zeng J Z, et al. Chill-induced inhibition of photosynjournal was alleviated by 24-epibrassinolide pretreatment in cucumber during chilling and subsequent recovery[J]. Photosynthetica, 2010,48(4):537-544. doi: 10.1007/s11099-010-0071-y URL
[34]	董轲. 低温光胁迫对植物光系统的伤害及缓解机理研究[D]. 济南:山东师范大学, 2017.
[35]	Gharechahi J, Alizadeh H, Naghavi M R, et al. A proteomic analysis to identify cold acclimation associatedproteins in wild wheat (Triticum urartu L.)[J]. Mol Biol Rep, 2014,41(6):3897-3905. doi: 10.1007/s11033-014-3257-8 URL pmid: 24535272
[36]	杨华庚, 林位夫. 低温胁迫对油棕幼苗光合作用及叶绿素荧光特性的影响[J]. 中国农学通报, 2009,25(24):506-509.

皂苷名称	生长年限	20℃	15℃	10℃
三七皂苷R₁	3年生	1.1002±0.1912a	1.2760±0.9310a	0.9799±0.2065a
三七皂苷R₁	2年生	0.2574±0.0432a	0.3646±0.0838a	0.2856±0.3160a
人参皂苷Rg₁	3年生	5.0023±1.3826a	3.8750±0.6021a	4.8011±1.0155a
人参皂苷Rg₁	2年生	1.8401±0.2009a	1.2949±0.1751b	1.7628±0.1373ab
人参皂苷Rb₁	3年生	3.9438±0.5614a	3.3774±1.1126a	3.1938±0.6631a
人参皂苷Rb₁	2年生	1.2188±0.1314a	1.1390±0.2073a	1.0020±0.1392a
人参皂苷Rd	3年生	1.2525±0.2324a	1.1082±0.3989a	1.0635±0.2502a
人参皂苷Rd	2年生	0.3249±0.0530a	0.3466±0.0903a	0.2913±0.0397a
总皂苷	3年生	11.2989±2.3231a	9.6366±1.8618a	10.0383±1.6252a
总皂苷	2年生	3.6412±0.2732a	3.1451±0.4100a	3.3416±0.2837a

皂苷名称	生长年限	20℃	15℃	10℃
三七皂苷R₁	3年生	1.1002±0.1912a	1.2760±0.9310a	0.9799±0.2065a
三七皂苷R₁	2年生	0.2574±0.0432a	0.3646±0.0838a	0.2856±0.3160a
人参皂苷Rg₁	3年生	5.0023±1.3826a	3.8750±0.6021a	4.8011±1.0155a
人参皂苷Rg₁	2年生	1.8401±0.2009a	1.2949±0.1751b	1.7628±0.1373ab
人参皂苷Rb₁	3年生	3.9438±0.5614a	3.3774±1.1126a	3.1938±0.6631a
人参皂苷Rb₁	2年生	1.2188±0.1314a	1.1390±0.2073a	1.0020±0.1392a
人参皂苷Rd	3年生	1.2525±0.2324a	1.1082±0.3989a	1.0635±0.2502a
人参皂苷Rd	2年生	0.3249±0.0530a	0.3466±0.0903a	0.2913±0.0397a
总皂苷	3年生	11.2989±2.3231a	9.6366±1.8618a	10.0383±1.6252a
总皂苷	2年生	3.6412±0.2732a	3.1451±0.4100a	3.3416±0.2837a

温度	生长年限	叶绿素a/(mg/g)	叶绿素b/(mg/g)	Pn/[μmolCO₂/(m²·s)]	Gs/[mmolH₂O/(m²·s)]	Tr/[mmolH₂O/(m²·s)]	MDA/[nmol/(g·FW)]	Ci/Ca
10℃	3年生	12.05±1.93a	10.40±0.42a	1.53±0.09c	0.020±0.0010b	0.253±0.012b	10.18±1.06a	0.68±0.00a
10℃	2年生	4.83±0.97a	2.33±0.79a	2.15±0.13a	0.026±0.0098a	0.224±0.053b	5.69±0.80a	0.65±0.08a
15℃	3年生	10.66±1.00a	8.01±0.86b	1.92±0.25b	0.022±0.0040b	0.253±0.049b	11.00±0.88	0.64±0.02b
15℃	2年生	6.27±0.97a	2.48±0.64a	2.38±0.49a	0.029±0.004a	0.207±0.038b	a5.41±0.31a	0.66±0.08a
20℃	3年生	10.76±1.32a	11.23±1.44a	2.37±0.18a	0.027±0.0010a	0.495±0.030a	9.71±1.30a	0.62±0.02b
20℃	2年生	4.75±1.64a	1.67±0.62a	2.55±0.14a	0.036±0.005a	0.393±0.090a	4.52±1.19a	0.70±0.04a

温度	生长年限	叶绿素a/(mg/g)	叶绿素b/(mg/g)	Pn/[μmolCO₂/(m²·s)]	Gs/[mmolH₂O/(m²·s)]	Tr/[mmolH₂O/(m²·s)]	MDA/[nmol/(g·FW)]	Ci/Ca
10℃	3年生	12.05±1.93a	10.40±0.42a	1.53±0.09c	0.020±0.0010b	0.253±0.012b	10.18±1.06a	0.68±0.00a
10℃	2年生	4.83±0.97a	2.33±0.79a	2.15±0.13a	0.026±0.0098a	0.224±0.053b	5.69±0.80a	0.65±0.08a
15℃	3年生	10.66±1.00a	8.01±0.86b	1.92±0.25b	0.022±0.0040b	0.253±0.049b	11.00±0.88	0.64±0.02b
15℃	2年生	6.27±0.97a	2.48±0.64a	2.38±0.49a	0.029±0.004a	0.207±0.038b	a5.41±0.31a	0.66±0.08a
20℃	3年生	10.76±1.32a	11.23±1.44a	2.37±0.18a	0.027±0.0010a	0.495±0.030a	9.71±1.30a	0.62±0.02b
20℃	2年生	4.75±1.64a	1.67±0.62a	2.55±0.14a	0.036±0.005a	0.393±0.090a	4.52±1.19a	0.70±0.04a