Study on the Correlation Between Serum Reproductive Hormone Content and Superovulation Effect in Dairy Meade Sheep

doi:10.11924/j.issn.1000-6850.casb2021-0518

Abstract

Abstract:

At present, superovulation of livestock still has the problems of large individual differences and unstable overall effect. The objective of this study is to explore the correlation between the reproductive hormones in serum of dairy Meade sheep and the superovulation effect of the donor, in order to provide a basis for the prediction of the superovulation performance of the donor. In this study, peripheral blood was collected to determine follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), progesterone (P4), anti-Müllerian hormone (AMH) and inhibin (INH) hormone concentrations before superovulation treatment and before mating, and to analyzed their correlations with the total number of embryos recovered and the number of available embryos. The results of blood test before superovulation showed that FSH, E2 and AMH (FSH: r=0.91, P<0.05; E2: r=0.76, P<0.05; AMH: r=0.86, P<0.05;) had a significantly positive correlation with the total number of embryos recovered and the number of available embryos; LH, P4 and INH (LH: r=-0.81, P<0.01; P4: r=-0.79, P<0.05; INH: r=-0.56, P<0.05;) had a significantly negative correlation with the total number of embryos recovered and the number of available embryos. The results of blood test before mating after superovulation showed that FSH, E2, P4 and AMH (FSH r=0.62, P<0.05; E2: r=0.93, P<0.05; P4: r=0.69, P<0.05; AMH: r=0.97, P<0.01) had a significantly positive correlation with the total number of embryos recovered and the number of available embryos; LH and INH (LH: r=-0.86, P<0.01; INH: r=-0.60, P<0.05) had a significantly negative correlation with the total number of embryos recovered and the number of available embryos. The conclusion of this study indicates that FSH, LH, E2, P4, AMH and INH in the venous blood of dairy Meade sheep before superovulation are all related to the final superovulation effect. The FSH and AMH hormone concentrations in the venous blood before superovulation have the highest fit with the total number of embryos and the number of available embryos, and there is a high correlation. Therefore, FSH and AMH can be used as indicators for the detection.

Key words: dairy Meade sheep, superovulation, reproductive hormones, correlation

CLC Number:

S814.6

GUO Yulin, ZHANG Liguo, ZHENG Zhong, WANG Chunwei, XU Quanzhong, WU Yunxi, LI Guangpeng, SU Xiaohu, ZHANG Li. Study on the Correlation Between Serum Reproductive Hormone Content and Superovulation Effect in Dairy Meade Sheep[J]. Chinese Agricultural Science Bulletin, 2022, 38(35): 90-96.

Figures/Tables 4

References 35

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组别	高产	中产	低产	总计
供体/只	8	10	5	23
回收总数/枚	155	120	14	289
范围	16-49	6~15	1~5	-
平均回收/枚	19.4	12	2.8	12.6
可用胚/枚	147	110	12	269
平均可用胚/枚	18.4	11	2.4	11.7
胚胎可用率/%	-	-	-	-

组别	高产	中产	低产	总计
供体/只	8	10	5	23
回收总数/枚	155	120	14	289
范围	16-49	6~15	1~5	-
平均回收/枚	19.4	12	2.8	12.6
可用胚/枚	147	110	12	269
平均可用胚/枚	18.4	11	2.4	11.7
胚胎可用率/%	-	-	-	-

激素名称	第一次			第二次
激素名称	高产组	中产组	低产组	高产组	中产组	低产组
FSH/(mIU/mL)	18.62±0.41a	17.05±0.15b	16.06±0.12c	19.29±0.25a	18.28±0.21b	18.10±0.15b
LH/(mIU/mL)	36.20±0.72b	35.35±0.84b	46.96±0.78a	49.14±0.59b	49.71±0.60b	53.78±1.15a
E2/(pg/mL)	236.39±6.4a	216.65±3.21b	212.23±2.8b	273.09±5.49a	257.58±5.24a	228.05±7.01b
P4/(ng/mL)	40.68±2.44b	45.81±0.32a	48.04±0.69a	68.64±4.54a	67.60±2.40a	58.51±3.70b
AMH/(ng/mL)	186.50±2.9a	177.90±0.87b	172.37±1.4b	242.08±6.25a	220.18±4.39b	179.51±5.69c
INH/(pg/mL)	63.48±0.76b	70.99±2.91a	74.09±6.75a	68.55±3.05b	83.37±3.09a	83.41±5.38a

激素名称	第一次			第二次
激素名称	高产组	中产组	低产组	高产组	中产组	低产组
FSH/(mIU/mL)	18.62±0.41a	17.05±0.15b	16.06±0.12c	19.29±0.25a	18.28±0.21b	18.10±0.15b
LH/(mIU/mL)	36.20±0.72b	35.35±0.84b	46.96±0.78a	49.14±0.59b	49.71±0.60b	53.78±1.15a
E2/(pg/mL)	236.39±6.4a	216.65±3.21b	212.23±2.8b	273.09±5.49a	257.58±5.24a	228.05±7.01b
P4/(ng/mL)	40.68±2.44b	45.81±0.32a	48.04±0.69a	68.64±4.54a	67.60±2.40a	58.51±3.70b
AMH/(ng/mL)	186.50±2.9a	177.90±0.87b	172.37±1.4b	242.08±6.25a	220.18±4.39b	179.51±5.69c
INH/(pg/mL)	63.48±0.76b	70.99±2.91a	74.09±6.75a	68.55±3.05b	83.37±3.09a	83.41±5.38a