The Contents and Anti-tumor Activity of Main Chemical Components of Scutellaria baicalensis from Different Origins: A Comparative Study

doi:10.11924/j.issn.1000-6850.casb2021-0248

Abstract

Abstract:

The purpose of the study is to investigate the effect of different origins on the contents of the chemical components and anti-tumor activity Scutellaria baicalensis. The study used HPLC chromatography to determine the contents of baicalin, wogonoside, baicalein and wogonin of S. baicalensis from different origins, and CCK-8 method to detect the inhibition of A549 lung cancer cell proliferation. The results show that there are significant differences in the contents of chemical components of S. baicalensis samples from different origins. The content of baicalin from high to low is Boli of Heilongjiang> Juxian of Shandong> Dingxi of Gansu> Yuncheng of Shanxi; the content of wogonoside from high to low is Boli of Heilongjiang> Dingxi of Gansu> Juxian of Shandong> Yuncheng of Shanxi; the content of baicalein from high to low is Boli of Heilongjiang> Juxian of Shandong> Yuncheng of Shanxi> Dingxi of Gansu; and the wogonin content from high to low is Boli of Heilongjiang> Yuncheng of Shanxi> Juxian of Shandong> Dingxi of Gansu. As shown above, the contents of the chemical components of S. baicalensis in Boli of Heilongjiang rank the first among those from the 4 origins. The extract of S. baicalensis has an inhibitory effect on A549 lung cancer cells, and the effect is related to the contents of the chemical components and dose-dependent. The experimental results can provide reference for the development and rational use of medicinal resources of S. baicalensis.

Key words: Scutellaria baicalensis, HPLC chromatography, baicalin, wogonoside, baicalein, wogonin, anti-tumor activity

CLC Number:

S182

FANG Jiamin, ZENG Weimin, ZHANG Yanlong. The Contents and Anti-tumor Activity of Main Chemical Components of Scutellaria baicalensis from Different Origins: A Comparative Study[J]. Chinese Agricultural Science Bulletin, 2022, 38(4): 120-126.

Figures/Tables 12

References 25

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样品编号	产地	生长方式	生长年限/年
S1	黑龙江勃利	栽培	2
S2	山东莒县	栽培	2
S3	山西运城	栽培	2
S4	甘肃定西	栽培	2

样品编号	产地	生长方式	生长年限/年
S1	黑龙江勃利	栽培	2
S2	山东莒县	栽培	2
S3	山西运城	栽培	2
S4	甘肃定西	栽培	2

时间/min	乙腈浓度/%	时间/min	乙腈浓度/%
0~10	8→12	70~73	63→63
10~20	12→17	73~77	63→70
20~30	17→24	77~80	70→80
30~60	24→34	80~85	80→8
60~70	34→63	85~90	8

时间/min	乙腈浓度/%	时间/min	乙腈浓度/%
0~10	8→12	70~73	63→63
10~20	12→17	73~77	63→70
20~30	17→24	77~80	70→80
30~60	24→34	80~85	80→8
60~70	34→63	85~90	8

溶剂名称	黄芩苷峰面积	汉黄芩苷峰面积	黄芩素峰面积	汉黄芩素峰面积
70%乙醇	50445983	10892585	5182270	1055412
80%甲醇	59529640	11976181	5919874	1111190
60%丙酮	36586637	8250972	3443369	1012837