Temperature and pH: Effect on the Elimination Rule of Enrofloxacin and Its Metabolite Ciprofloxacin in Fishery Lower Water Bodies

doi:10.11924/j.issn.1000-6850.casb20200100022

Abstract

Abstract:

The aim is to investigate the elimination patterns of enrofloxacin in the lower water column of the fishery and the production of the metabolite ciprofloxacin, since antibiotics entering the water column are prone to accumulate in the lower water column or in the substrate sediment, and cause adverse effects on aquatic life. Common environmental factors (temperature and pH) in the fishery water were selected for the experiment, and effects of these two environmental factors on the degradation of enrofloxacin (ENR) in aqueous humor were investigated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with orthogonal experiments, and the production and changes of the metabolite ciprofloxacin (CIP) were detected. The experimental results showed that the half-life variation of ENR in the nine treatment groups ranged from 13 to 32 d and the elimination rate ranged from 44% to 80%. Root ANOVA knots showed that pH changes had a significant effect on the degradation of enrofloxacin, while temperature changes did not have a significant effect, with the highest elimination rate of enrofloxacin at 25°C and pH 5. In addition, the production of ciprofloxacin was detected during the experiment, which reached the highest concentration (10.8 ng/mL) at pH 5 and 25°C on day 7 from the beginning of the experiment, and then gradually decreased to the concentration 0-0.16 ng/mL on day 26. This study clarifies the influence of environmental factors on enrofloxacin degradation and provides some theoretical guidance for reducing enrofloxacin residues in aquaculture environment.

Key words: enrofloxacin, ciprofloxacin, lower water body, environmental factors, eliminate rule

CLC Number:

S948

Huang Zhuyu, Fang Longxiang, Song Chao, Mussa Ramadhan Said, Yang Guang, Li Tian, Chen Jiazhang. Temperature and pH: Effect on the Elimination Rule of Enrofloxacin and Its Metabolite Ciprofloxacin in Fishery Lower Water Bodies[J]. Chinese Agricultural Science Bulletin, 2020, 36(21): 132-139.

Figures/Tables 8

References 37

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时间/min	流速/(mL/min)	φ(A)/%	φ(B)/%
初始	0.3	80	20
2.50	0.3	70	30
2.60	0.3	68	32
4.00	0.3	30	70
4.50	0.3	80	20
6.00	0.3	80	20

时间/min	流速/(mL/min)	φ(A)/%	φ(B)/%
初始	0.3	80	20
2.50	0.3	70	30
2.60	0.3	68	32
4.00	0.3	30	70
4.50	0.3	80	20
6.00	0.3	80	20

化合物名称	母离子(m/z)	子离子(m/z)	锥孔电压/V	碰撞能量/V	保留时间/min
恩诺沙星	360.26	72.17^*	48	30	2.68
恩诺沙星	360.26	84.20	48	30	2.68
环丙沙星	332.23	231.50	50	30	2.58
环丙沙星	332.23	245.17^*	50	22	2.58

化合物名称	母离子(m/z)	子离子(m/z)	锥孔电压/V	碰撞能量/V	保留时间/min
恩诺沙星	360.26	72.17^*	48	30	2.68
恩诺沙星	360.26	84.20	48	30	2.68
环丙沙星	332.23	231.50	50	30	2.58
环丙沙星	332.23	245.17^*	50	22	2.58

消除率	自由度 df	Seq SS	Adj SS	Adj MS	F	P
pH	2	0.17695	0.17695	0.08847	7.48	0.003
温度	2	0.08109	0.08109	0.04054	3.43	0.051