Abstract:

To make full use of the insecticidal and antibacterial ingredients in peony seed meal, and explore the effect of different concentrations of extract on the continuous cropping soil, this study combined single factor and orthogonal experiment, soil chemical property analysis and laboratory biological active determination methods against Gaeumannomyces graminis, to determine that 60% ethanol concentration, 120 W ultrasonic power, and 50℃ temperature was the optimal extraction condition of peony seed meal effective ingredients. At the same time, in a certain concentration range, the peony seed meal extract could reduce the pH of continuous cropping soil, improve soil alkalinity, and show a “low promotion and high inhibition” effects on the organic matter content, total nitrogen, carbon to nitrogen ratio, total phosphorus and ash content. The peony seed meal extract was also found to be beneficial to increase the fungi and actinomycetes number and cellulase and urease activities. In addition, in vitro antifungal activity against Gaeumannomyces graminis with peony seed meal extract T4 (0.8 mg/mL) had a good efficacy, which could reach 63.42%. The results of this study could lay a foundation for the resource utilization of peony seed meal and the development of natural environmentally friendly fungicides.

Key words: peony seed meal, orthogonal experiment, extraction rate, continuous cropping soil, Gaeumannomyces graminis, control effect